PMID: 2505675Oct 1, 1989Paper

Preparative in vitro generation of lacto-series type 1 chain glycolipids catalyzed by beta 1----3-galactosyltransferase from human colonic adenocarcinoma Colo 205 cells

Archives of Biochemistry and Biophysics
E H Holmes, S B Levery

Abstract

Lacto-series glycolipids, comprising two isomeric types distinguished as type 1 or 2 based upon the linkage of the terminal galactose of the chains, form the basis for a diversity of cell surface antigens expressed on cells. Experimentally, type 2 chain precursors are generally more abundant in tissues for extractive purposes to yield rather large quantities of material compared to the type 1 chain structures. Conditions have been defined for in vitro conversion of terminal Gal beta 1----4GlcNAc linkages of type 2 chain precursors to yield type 1 lacto-series chain based terminal Gal beta 1----3GlcNAc structures in 5- to 10-mg amounts or higher. The terminal galactose of underivatized type 2 chain structures is removed by hydrolysis with jack bean beta-galactosidase followed by transfer of galactose in beta 1----3 linkage catalyzed by a beta 1----3-galactosyltransferase from human colonic adenocarcinoma Colo 205 cells which was first depleted of beta 1----4-galactosyltransferase by chromatography on alpha-lactalbumin-Sepharose. Scaled-up reaction mixtures provided a final yield of product after isolation of about 90% from the immediate Lc3Cer precursor in the 5-mg product range. The biosynthetic product was subjected to extensi...Continue Reading

References

Dec 15, 1986·Chemistry and Physics of Lipids·S Hakomori
May 1, 1985·Chemistry and Physics of Lipids·H EggeG Tettamanti
May 15, 1974·European Journal of Biochemistry·B S KhatraK Brew
Jan 1, 1973·Hoppe-Seyler's Zeitschrift für physiologische Chemie·W Stoffel, P Hanfland
Jan 1, 1984·Annual Review of Immunology·S Hakomori
Dec 16, 1983·Biochemical and Biophysical Research Communications·M RanceK Wüthrich

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Citations

Oct 1, 1994·Journal of Neuroscience Research·J LiA J Yates
Mar 30, 2001·The Journal of Biological Chemistry·A ItoS Hakomori
Mar 27, 2002·The Journal of Biological Chemistry·Susann TenebergKarl-Anders Karlsson
Nov 1, 1992·The Journal of Investigative Dermatology·F W SymingtonB E Symington

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