Preparative isolation and purification of amides from the fruits of Piper longum L. by upright counter-current chromatography and reversed-phase liquid chromatography

Journal of Chromatography. a
Shihua WuYuanjiang Pan

Abstract

A versatile counter-current chromatography (CCC) with upright type-J multilayer coil planet centrifuge, named upright CCC, was applied to the isolation and purification of amides from Piper longum L., which is widely used as an anodyne and a treatment for stomach disease in China. After the saponification by KOH of the ethanol extracts solution of 15 kg of crude drug "Piper Longi Fructus", the fruits of P. longum L., the solution was extracted with light petroleum and 500 g of red crude oil was obtained. Using 2.5 g of red crude oil as sample, the preparative upright CCC with a two-phase system composed of light petroleum (bp 60-90 degrees C)-ethyl acetate-tetrachloromethane-methanol-water (1:1:8:6:1, v/v) was successfully performed, which yielded nine fractions. Then these fractions were further purified by use of reversed-phase liquid chromatography (RPLC) with a glass column of 500 x 10 mm i.d. packed with reversed-phase silica gel. As a result, nine target amides with over 95% purity, i.e., 50 mg of (2E,4E)-N-isobutyl-eicosa-2,4-dienamide, 150 mg of (2E,4E,14Z)-N-isobutyl-eicosa-2,4,14-trienamide, 110 mg of (2E,4E,12Z)-N-isobutyl-ocatadeca-2,4,12-trienamide, 50 mg of guineensine, 60 mg of pipernonaline, 75 mg of pellitorine...Continue Reading

References

Feb 21, 2002·Phytochemistry·Renata Vasques da SilvaMaysa Furlan

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Citations

Jun 20, 2008·Archives of Pharmacal Research·Seon A LeeBang Yeon Hwang
Nov 26, 2011·Natural Product Research·Saksit NobsathianChanita Napaswad
Aug 13, 2013·Journal of Natural Medicines·Kartik Chandra PatraDheeraj Kumar Ahirwar
Aug 11, 2012·Bioscience, Biotechnology, and Biochemistry·Jiachen ZiJixun Zhan
Jun 15, 2016·BMC Complementary and Alternative Medicine·Lieselotte VeryserBart De Spiegeleer
Jun 5, 2012·Journal of Ethnopharmacology·Jente BoonenBart De Spiegeleer
Jan 28, 2009·Phytotherapy Research : PTR·Zhuang JinToshiyuki Uryu
May 11, 2019·Natural Products and Bioprospecting·Rong TangYue-Hu Wang
Nov 28, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Juan David Guzman
Dec 15, 2004·Phytochemical Analysis : PCA
May 31, 2018·Natural Product Research·Hong-Zhang DiaoChang-Ri Han
May 3, 2019·The Journal of Pharmacy and Pharmacology·Ziyan GuoJianqing Yu
Jun 5, 2021·Journal of Asian Natural Products Research·Chalini NongmaiSomdej Kanokmedhakul
Feb 23, 2012·Journal of Agricultural and Food Chemistry·Corinna DawidThomas Hofmann
Apr 5, 2008·Journal of Agricultural and Food Chemistry·Mendel FriedmanNobuyuki Kozukue

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