Preparative isolation and purification of two benzoxazinoid glucosides from Acanthus ilicifolius L. by high-speed counter-current chromatography

Journal of Chromatography. a
Hao YinYonghong Liu

Abstract

The first preparative separation of two benzoxazinoids, (2R)-2-O-beta-d-glucopyranosyl-2H-1,4-benzoxazin-3(4H)-one (HBOA-Glc) and (2R)-2-O-beta-d-glucopyranosyl-4-hydroxy-2H-1,4-benzoxazin-3(4H)-one (DIBOA-Glc), by means of high-speed counter-current chromatography (HSCCC) from the n-butanol extract of Acanthus ilicifolius L. is presented. The two-phase solvent system containing ethyl acetate-n-butanol-0.5%NH(4)OH (2:3:5, v/v/v, system B) was selected for the one-step HSCCC separation of HBOA-Glc and DIBOA-Glc according to the partition coefficient values (K) for target compounds and the separation factor (alpha) between the two target compounds. In the one-step HSCCC separation using solvent B, from 100mg n-butanol extract of A. ilicifolius, 6.3 mg HBOA-Glc and 6.8 mg DIBOA-Glc were isolated with purities of 90.3% and 80.2%, respectively. In order to obtain the two target compounds with higher purity, a second separation process was developed comprising two steps. In the two-step separation, the sample was first pre-purified by HSCCC using ethyl acetate-n-butanol-water (2:3:5, v/v/v, system A) solvent system and then purified using solvent system B. A 100-mg amount of the n-butanol extracts of A. ilicifolius was separated to y...Continue Reading

Citations

Jul 16, 2015·Journal of Natural Products·J Brent FriesenGuido F Pauli
Sep 8, 2011·Journal of Separation Science·Lei FangLuqi Huang
Jul 12, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lifeng HanXiumei Gao
Jan 5, 2010·Journal of Liquid Chromatography & Related Technologies·Xiuyun JuYoichiro Ito
Apr 15, 2011·Journal of Separation Science·Zhao-Hui YangXiao-Mei Ren
Jul 28, 2013·Journal of Agricultural and Food Chemistry·Cammy D WillettCraig A Roberts

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