Preparative isolation of arylbutanoid-type phenol [(-)-rhododendrin] with peak tailing on conventional C18 column using middle chromatogram isolated gel column coupled with reversed-phase liquid chromatography.

Journal of Separation Science
Jun DangYanduo Tao

Abstract

Reversed-phase liquid chromatography coupled with middle chromatogram isolated gel column was employed for the efficient preparative separation of the arylbutanoid-type phenol [(-)-rhododendrin] from Saxifraga tangutica. Universal C18 (XTerra C18) and XCharge C18 columns were compared for (-)-rhododendrin fraction analysis and preparation. Although tailing and overloading occurred on the XTerra C18 column, the positively charged reversed-phase C18 column (XCharge C18) overcame these drawbacks, allowing for favorable separation resolution, even when loading at a on a preparative scale (3.69 mg per injection). The general separation process was as follows. First, 365.0 mg of crude (-)-rhododendrin was enriched from 165 g Saxifraga tangutica extract via a middle chromatogram isolated gel column. Second, separation was performed on an XTerra C18 preparative column, from which 73.8 mg of the target fraction was easily obtained. Finally, the 24.0 mg tailing peak of (-)-rhododendrin on XTerra C18 column was selectively purified on the XCharge C18 analytical column. These results demonstrate that the tailing nonalkaloid peaks can be effectively used for preparative isolation on XCharge C18 columns.

References

Dec 7, 2005·Antiviral Chemistry & Chemotherapy·Guo-Ying ZuoXiao-Jie Xu
Mar 23, 2010·Natural Product Research·Mahmoud A Al-Qudah, Musa H Abu Zarga
Feb 16, 2013·Journal of Chromatography. a·Chaoran WangXinmiao Liang
Mar 21, 2013·Journal of Chromatographic Science·Santaji Nalwade, Vangala Ranga Reddy
Feb 8, 2014·Journal of Pharmaceutical and Biomedical Analysis·Xiaolong LiXinmiao Liang
Feb 16, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Nusrat ShaheenYun Wei
May 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Weicheng HuXinfeng Wang

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