Preparative purification of tetraantennary oligosaccharides from human asialyl orosomucoid

Analytical Biochemistry
H J StubbsK G Rice

Abstract

An approach to isolate micromole quantities of tetraantennary oligosaccharides from human orosomucoid is presented. The N-linked oligosaccharides from 500 mg of the glycoprotein were released enzymatically, desialylated, and isolated free of protein using ion exchange chromatography. The pooled oligosaccharides were converted into oligosaccharide glycosylamines by reaction with ammonium bicarbonate then coupled to BOC-tyrosine to prepare tyrosinamide oligosaccharides. These were resolved on semipreparative RP-HPLC to recover micromole quantities of six purified tyrosinamide oligosaccharides. The oligosaccharide structures were elucidated by a combination of high-field proton NMR and matrix-assisted time of flight mass spectrometry and included biantennary, triantennary, monofucosylated triantennary, tetraantennary, monofucosylated tetraantennary, and a tetraantennary containing a single polylactosamine extension. Edman degradation was utilized to reverse the tyrosinamide oligosaccharide derivatization leading to the generation of reducing oligosaccharides. These were used to characterize the elution profile of asialyl orosomucoid oligosaccharides on high pH anion exchange chromatography. This application of tyrosinamide derivat...Continue Reading

References

Jun 24, 1977·Biochimica Et Biophysica Acta·K SchmidJ P Binette
May 15, 1988·Analytical Biochemistry·N TomiyaN Takahashi
Feb 1, 1994·Analytical Biochemistry·T TamuraK G Rice
Jan 1, 1994·Methods in Enzymology·T TamuraK G Rice
May 1, 1995·Bioconjugate Chemistry·M S WadhwaK G Rice
Apr 20, 1995·Archives of Biochemistry and Biophysics·M L Corradi Da SilvaK G Rice
Oct 1, 1994·Analytical Biochemistry·T McBroomK G Rice
Dec 1, 1994·Archives of Biochemistry and Biophysics·M L Corradi da SilvaK G Rice
Jan 1, 1993·Annual Review of Biochemistry·R A DwekR B Parekh
Jan 12, 1996·Journal of Chromatography. a·K G Rice, M L Corradi Da Silva
May 1, 1997·Analytical Biochemistry·W T CollardK G Rice

❮ Previous
Next ❯

Citations

Mar 13, 2010·Analytical and Bioanalytical Chemistry·L R RuhaakM Wuhrer
Mar 12, 2011·Journal of Mass Spectrometry : JMS·Yu-Ling ChangYuan Chuan Lee
Apr 15, 2004·Bioorganic & Medicinal Chemistry Letters·Kiichiro TotaniYukishige Ito
Nov 9, 2017·Bioscience, Biotechnology, and Biochemistry·Maiko TanaseHideharu Ishida
Aug 10, 2000·Analytical Biochemistry·K G Rice
May 1, 1997·Analytical Biochemistry·W T CollardK G Rice
Nov 13, 2004·Rapid Communications in Mass Spectrometry : RCM·David J HarveyChris W Sutton
Jul 28, 2021·Current Protocols·Noriko Suzuki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.