Preparative separation and purification of epigallocatechin gallate from green tea extracts using a silica adsorbent containing β-cyclodextrin

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Shih-Ming LaiWen-Lung Lee

Abstract

A silica adsorbent containing β-cyclodextrin (β-CD) has been developed and used for the separation and purification of epigallocatechin gallate (EGCG) from the green tea extracts. The batch adsorption experiments demonstrated that, the β-CD bonded silica adsorbent possessed excellent adsorption equilibrium capacity (> 55 mg/g adsorbent) and adsorption ratio (>95%) for EGCG compared to the other tea catechins and caffeine. The excellent adsorption capacity and selectivity for EGCG are attributed to the specific interactions between β-CD and EGCG. The preparative separation and purification performance of EGCG on the β-CD bonded silica column (220 mm L × 15 mm i.d., 40-63 μm) was then evaluated. The column was operated in the polar organic mode using methanol/acetonitrile/acetic acid as the mobile phase and eluted under a three-step gradient elution program. The sample was dissolved in acetonitrile and loaded on a preparative scale of about 0.8 mg/g adsorbent. Under the optimal chromatographic conditions, the target compound, EGCG, being the most retained species, was obtained at a purity of about 90% with a recovery of about 90%. The productivity of EGCG was about 6 mg per injection, which can be further increased by scaling-up ...Continue Reading

Citations

Jun 2, 2015·Journal of Food Science and Technology·Satarupa Banerjee, Jyotirmoy Chatterjee
Dec 31, 2020·Nanomaterials·Carolina Belenguer-SapiñaPedro Amorós
May 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Mehdi NikooHassan Ahmadi Gavlighi

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