Preparative Separation and Purification of Trichothecene Mycotoxins from the Marine Fungus Fusarium sp. LS68 by High-Speed Countercurrent Chromatography in Stepwise Elution Mode

Marine Drugs
Yong LiuShan He

Abstract

The contamination of foods and animal feeds with trichothecene mycotoxins is a growing concern for human and animal health. As such, large quantities of pure trichothecene mycotoxins are necessary for food safety monitoring and toxicological research. A new and effective method for the purification of trichothecene mycotoxins from a marine fungus, Fusarium sp. LS68, is described herein. Preparative high-speed countercurrent chromatography (HSCCC) was utilized for the scalable isolation and purification of four trichothecene mycotoxins for the first time in stepwise elution mode, with a biphasic solvent system composed of hexanes-EtOAc-CH₃OH-H₂O (6:4:5:5, v/v/v/v) and (8.5:1.5:5:5,v/v/v/v). This preparative HSCCC separation was performed on 200 mg of crude sample to yield four trichothecene mycotoxins, roridin E (1), roridin E acetate (2), verrucarin L acetate (3), and verrucarin J (4) in a single run, with each of >98% purity. These compounds were identified by MS, ¹H NMR, 13C NMR, and polarimetry. The results demonstrate an efficient HSCCC method for the separation of trichothecene mycotoxins, which can be utilized to produce pure commercial and research standards.

References

Feb 17, 2007·Fortschritte der Chemie organischer Naturstoffe = Progress in the chemistry of organic natural products. Progrès dans la chimie des substances organiques naturelles·J F Grove
Apr 25, 2007·Fungal Genetics and Biology : FG & B·David E StarkeyKerry O'Donnell
Jul 17, 2007·Journal of Agricultural and Food Chemistry·Anne E DesjardinsMichael Appell
May 24, 2008·Natural Product Reports·Otto Sticher
Dec 26, 2008·Journal of Chromatography. a·Ian A Sutherland, Derek Fisher
Nov 1, 2004·Molecular Plant Pathology·Rubella S Goswami, H Corby Kistler
Jan 1, 2010·Journal of Liquid Chromatography & Related Technologies·Yi YangHaji Akber Aisa
Oct 11, 2012·Molecular Nutrition & Food Research·Franz BerthillerJoerg Stroka
Jul 15, 2015·Journal of Agricultural and Food Chemistry·Zdenka VeprikovaJana Hajslova
Oct 14, 2016·Magnetic Resonance in Chemistry : MRC·Clark D RidgeSimon F R Hinkley
May 12, 2017·Journal of Agricultural and Food Chemistry·Seung Mok RyuDongho Lee
Dec 1, 1997·Plant Disease·Marcia McMullenDale Gallenberg

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