Preparing polymer-based sustained-release systems without exposing proteins to water-oil or water-air interfaces and cross-linking reagents

Journal of Controlled Release : Official Journal of the Controlled Release Society
Tuo JinHua Zhu

Abstract

We report a method to load proteins into polymer-based sustained-release systems without exposing them to water-oil or water-air interfaces, factors known to denature proteins. By dispersing a dextan solution containing a protein into a PEG solution containing small amount of alginate, a stable aqueous-aqueous "emulsion" was formed. The poly-anionic alginate generated a diffuse double layer around each dextran droplet to prevent them from contacting with each other and fusing to a block phase. Proteins distributed in the stabilized dextran droplets by preferential partition favoring dextran. Freeze-drying this emulsion resulted in protein-loaded dextran particles, 1-2 microm in diameter and 1.6 g/cm(3) in density. The particles were harvested by washing the lyophilized powder using organic solvents to remove the PEG continuous phase. An activity assay of encapsulated beta-galactosidase indicated that protein activity was preserved during the particle-forming process including the step of sonicating the particles in organic solvents. The dextran particles also improved release profile and integrity of proteins when encapsulated in degradable polymer sustained-release systems. The aqueous-aqueous emulsion offers a convenient way ...Continue Reading

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Citations

Apr 15, 2011·Journal of Drug Delivery·Maluta S MufamadiValence M K Ndesendo
Aug 28, 2012·International Journal of Nanomedicine·Guang LiuWeien Yuan
Sep 17, 2013·International Journal of Nanomedicine·Dan XuWeien Yuan
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May 3, 2014·Viruses·Han ZhangBao-Zhong Wang
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Aug 12, 2009·Expert Opinion on Drug Delivery·Sixing YangTuo Jin
May 31, 2011·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Xianfang RongXin Wang
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Nov 26, 2008·Biomaterials·Byung Soo KimMoon Suk Kim
Oct 7, 2008·International Journal of Pharmaceutics·Weien YuanTuo Jin
Apr 5, 2011·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Adam W Martinez, Elliot L Chaikof
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Aug 31, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Li WangBao-Zhong Wang
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Oct 27, 2018·Expert Review of Vaccines·Ye WangBao-Zhong Wang
Nov 27, 2018·Pharmaceutical Development and Technology·Misbah Tabbassum, Farrukh Zeeshan
Nov 6, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kinam ParkYan Wang
Apr 9, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fei WuTuo Jin

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