Preprocessing, normalization and integration of the Illumina HumanMethylationEPIC array

BioRxiv : the Preprint Server for Biology
Jean-Philippe FortinKasper D Hansen

Abstract

The minfi package is widely used for analyzing Illumina DNA methylation array data. Here we describe modifications to the minfi package required to support the HumanMethylationEPIC ("EPIC") array from Illumina. We discuss methods for the joint analysis and normalization of data from the HumanMethylation450 ("450k") and EPIC platforms. We also introduce the single-sample Noob method, a normalization procedure suitable for incremental preprocessing of individual HumanMethylation arrays. Our results recommend the single sample Noob method when integrating data from multiple generations of Infinium methylation arrays. Finally, we show how to use reference 450k datasets to estimate cell type composition of samples on EPIC arrays. The cumulative effect of these updates is to ensure that minfi provides the tools to best integrate existing and forthcoming Illumina methylation array data.

Related Concepts

DNA Methylation [PE]
Protein Methylation
DNA Methylation
Joints
Normalize
Methylation
Epithelial Cell Count (Procedure)
Cell Type
Analysis
Sampling Method

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