Presence and functionality of mating type genes in the supposedly asexual filamentous fungus Aspergillus oryzae.

Applied and Environmental Microbiology
Ryuta WadaKatsuhiko Kitamoto

Abstract

The potential for sexual reproduction in Aspergillus oryzae was assessed by investigating the presence and functionality of MAT genes. Previous genome studies had identified a MAT1-1 gene in the reference strain RIB40. We now report the existence of a complementary MAT1-2 gene and the sequencing of an idiomorphic region from A. oryzae strain AO6. This allowed the development of a PCR diagnostic assay, which detected isolates of the MAT1-1 and MAT1-2 genotypes among 180 strains assayed, including industrial tane-koji isolates. Strains used for sake and miso production showed a near-1:1 ratio of the MAT1-1 and MAT1-2 mating types, whereas strains used for soy sauce production showed a significant bias toward the MAT1-2 mating type. MAT1-1 and MAT1-2 isogenic strains were then created by genetic manipulation of the resident idiomorph, and gene expression was compared by DNA microarray and quantitative real-time PCR (qRT-PCR) methodologies under conditions in which MAT genes were expressed. Thirty-three genes were found to be upregulated more than 10-fold in either the MAT1-1 host strain or the MAT1-2 gene replacement strain relative to each other, showing that both the MAT1-1 and MAT1-2 genes functionally regulate gene expression ...Continue Reading

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Citations

Oct 3, 2013·Applied Microbiology and Biotechnology·Ulrich Kück, Julia Böhm
Nov 10, 2013·Applied Microbiology and Biotechnology·Ryuta WadaKatsuhiko Kitamoto
Jan 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Julia BöhmUlrich Kück
Jul 10, 2013·Applied and Environmental Microbiology·A J HunterJ M Kelly
May 8, 2013·Eukaryotic Cell·Sameira S SwilaimanPaul S Dyer
Mar 22, 2014·PloS One·P Markus WilkenBrenda D Wingfield
Aug 15, 2014·Mycopathologia·János VargaPaul S Dyer
Mar 30, 2016·Fungal Biology·Tuan A DuongMichael J Wingfield
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Apr 26, 2015·Fungal Genetics and Biology : FG & B·A M WilsonB D Wingfield
Mar 13, 2015·Frontiers in Microbiology·Ana M Calvo, Jeffrey W Cary
Sep 18, 2014·Bioscience, Biotechnology, and Biochemistry·Wakako TsukasakiKatsuhiko Kitamoto
Feb 6, 2017·Mycobiology·Anbazhagan MageswariSeung-Beom Hong
Feb 20, 2019·Bioscience, Biotechnology, and Biochemistry·Noriko MoriJun-Ichi Maruyama
Jan 20, 2019·BMC Genomics·Morgane Le MarquerNicolas Frei Dit Frey
Nov 23, 2019·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Naoki WataraiTakuji Yamada
Feb 19, 2021·Frontiers in Microbiology·Stephanie van WykEmma T Steenkamp
Jun 10, 2017·Microbiology Spectrum·Paul S Dyer, Ulrich Kück
Nov 15, 2018·Studies in Mycology·M Ojeda-LópezP S Dyer

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