PMID: 7019219Jun 1, 1981Paper

Presence and indirect immunofluorescent localization of calmodulin in Paramecium tetraurelia

The Journal of Cell Biology
N J MaihleB H Satir

Abstract

In this paper we demonstrate the presence and localization of calmodulin, a calcium-dependent regulatory protein, in the ciliated protozoan Paramecium tetraurelia. Calmodulin is demonstrated by several criteria: (a) the ability of whole cell Paramecium extracts to stimulate mammalian phosphodiesterase activity, (b) the presence of an acidic, thermostable, 17,000-dalton polypeptide whose mobility shifts in SDS polyacrylamide gel electrophoresis in the presence of Ca2+, and (c) the affinity of antibodies against mammalian calmodulin for a Paramecium component as demonstrated by both indirect immunofluorescent localization and radioimmunoassay. Indirect immunofluorescence studies reveal that Paramecium calmodulin is distributed in three distinct regions of the cell, i.e., (a) large, spherical cytoplasmic organelles representing perhaps the food vacuoles or other vacuolar inclusions of the cell, (b) along the entire length of oral and somatic cilia, and (c) along a linear punctate pattern corresponding to the kinetics (basal bodies) of the cell.

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Citations

Jan 1, 1988·Trends in Neurosciences·R D Hinrichsen, J E Schultz
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A G Moss, S L Tamm
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S L Tamm, S Tamm
Jun 1, 1985·The Journal of Cell Biology·C J Brokaw, S M Nagayama
Apr 1, 1991·The Journal of Cell Biology·N StellyA Adoutte
Jun 1, 1994·The Journal of Cell Biology·S L Tamm, M Terasaki
Dec 1, 1981·The Journal of Cell Biology·J J Rauh, D L Nelson
Mar 1, 1982·The Journal of Cell Biology·E W StommelJ F Head
Oct 1, 1982·The Journal of Cell Biology·R E GordonS Puszkin
Apr 1, 1983·The Journal of Cell Biology·R S GarofaloB H Satir
Jul 1, 1983·The Journal of Cell Biology·D M Gilligan, B H Satir
Oct 21, 2009·FEBS Letters·Felix BemmJörg Schultz
Apr 1, 1985·Experimental Cell Research·J Hirano, Y Watanabe
Jul 1, 1989·The Journal of protozoology·B H Satir
Jan 1, 1982·Progress in Clinical and Biological Research·K Takahashi
Apr 1, 1988·Journal of Cellular Biochemistry·B H SatirT J Murtaugh
Jan 1, 1986·Journal of Cellular Biochemistry·N J Maihle, B H Satir
Oct 22, 2015·Biological Reviews of the Cambridge Philosophical Society·Helmut Plattner
Jan 1, 1980·Annals of the New York Academy of Sciences·N J Maihle, B H Satir
Jan 1, 1980·Annals of the New York Academy of Sciences·B H SatirN J Maihle

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