Presence and infectivity of SARS-CoV-2 virus in wastewaters and rivers

The Science of the Total Environment
Sara Giordana RimoldiFranco Salerno

Abstract

The presence of SARS-CoV-2 in raw wastewaters has been demonstrated in many countries affected by this pandemic. Nevertheless, virus presence and infectivity in treated wastewaters, but also in the receiving water bodies are still poorly investigated. In this study, raw and treated samples from three wastewater treatment plants, and three river samples within the Milano Metropolitan Area, Italy, were surveyed for SARS-CoV-2 RNA detection by means of real time RT-PCR and infectivity test on culture cells. SARS-CoV-2 RNA was detected in raw, but not in treated wastewaters (four and two samples, respectively, sampled in two dates). The isolated virus genome was sequenced, and belonged to the strain most spread in Europe and similar to another found in the same region. RNA presence in raw wastewater samples decreased after eight days, probably following the epidemiological trend estimated for the area. Virus infectivity was always null, indicating the natural decay of viral pathogenicity in time from emission. Samples from receiving rivers (three sites, sampled in the same dates as wastewaters) showed in some cases a positivity to real time RT-PCR, probably due to non-treated, or inefficiently treated discharges, or to the combined...Continue Reading

Citations

Sep 8, 2020·Transboundary and Emerging Diseases·Isabel G Fernández-de-MeraChristian Gortázar
Sep 1, 2020·Water Environment Research : a Research Publication of the Water Environment Federation·Rasha Maal-BaredAlbert Rubin
Dec 7, 2020·Briefings in Bioinformatics·Matteo ChiaraGraziano Pesole
Nov 23, 2020·The Science of the Total Environment·Akihiko HataRyo Honda
Dec 19, 2020·The Science of the Total Environment·Alba Pérez-CataluñaGloria Sánchez
Dec 4, 2020·The Science of the Total Environment·Enric Cuevas-FerrandoGloria Sánchez
Nov 26, 2020·Trends in Analytical Chemistry : TRAC·Nikiforos AlygizakisNikolaos S Thomaidis
Dec 29, 2020·The Science of the Total Environment·Kouichi KitamuraHiromu Yoshida
Oct 5, 2020·Environmental Research·Hai Nguyen TranHuan-Ping Chao
Dec 8, 2020·Journal of Environmental Chemical Engineering·Bhavini Saawarn, Subrata Hait
Aug 25, 2020·The Science of the Total Environment·Giuseppina La RosaElisabetta Suffredini
Oct 7, 2020·The Science of the Total Environment·Mohamed ElsamadonyToru Watanabe
Oct 5, 2020·The Science of the Total Environment·Jie Han, Shanshan He
Feb 9, 2021·The Science of the Total Environment·Sabela BalboaJuan M Lema
Dec 17, 2020·International Journal of Environmental Research and Public Health·Maria de Lourdes Aguiar-OliveiraMarilda M Siqueira
Feb 1, 2021·Chemosphere·Sahar GholipourHossein Mirhendi
Jan 15, 2021·Chemosphere·Ernestine AtanganaAnthony R Turton
Feb 18, 2021·Reviews on Environmental Health·Hatam GodiniHooshyar Hossini
Feb 20, 2021·The Science of the Total Environment·Erick R BandalaAshantha Goonetilleke
Feb 22, 2021·The Science of the Total Environment·Alexandre GiacobboMaria Norberta de Pinho
Mar 2, 2021·Chemical Engineering Journal·Xuan LiGuangming Jiang
Feb 23, 2021·One Health·Inês FronteiraPaulo Ferrinho
Dec 30, 2020·The Science of the Total Environment·Dasheng LiuXuejun Bi
Mar 15, 2021·The Science of the Total Environment·Marion DesdouitsFrançoise S Le Guyader
Mar 19, 2021·Journal of Hazardous Materials·Antonio BuonerbaVincenzo Naddeo
Mar 20, 2021·Environmental Science and Pollution Research International·Mohammad TanhaeiMohammad Reza Zali
Mar 20, 2021·The Science of the Total Environment·Manupati HemalathaRakesh K Mishra
Mar 24, 2021·International Journal of Environmental Health Research·Omar AmahmidDriss Belghyti
Apr 4, 2021·Membranes·Rafał Tytus BrayAleksandra Sokołowska

❮ Previous
Next ❯

Datasets Mentioned

BETA
MN908947.3

Methods Mentioned

BETA
PCR
of

Software Mentioned

RaxML8
R library Ape
Trimmomatic
NG
ModelTest
iTol

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.