Presence and localization of aquaporin-6 in rat parotid acinar cells

Cell and Tissue Research
Miwako Matsuki-FukushimaH Sugiya

Abstract

Aquaporins (AQPs) are integral membrane proteins that function as channels for the transfer of water and small solutes across membranes. In mammalian cells, 13 isoforms (AQP0-12) have been identified, and these exhibit unique patterns of expression in various cell types and tissues. Among these isoforms, AQP6 is considered to function not as water channel, but as an anion channel. We investigated the presence and localization of AQP6 in rat parotid acinar cells. AQP6 mRNA was detected in these cells by using reverse transcription/polymerase chain reaction, and Western blotting analysis identified a protein band that reacted with an anti-AQP6 antibody in the membrane fraction and secretory granule membrane. In order to localize AQP6, we used the anti-AQP6 antibody for histological immunodetection. Under confocal microscopy, we observed positive immunoreactions near the tight junctions of parotid acinar cells. Immunolabeling of ultrathin cryosections detected AQP6 near tight junctions and around secretory granule membranes. Immunoelectron microscopy confirmed the presence of AQP6 in the membranes of isolated secretory granules. These results suggest that AQP6 participates in water and anion transport in plasma membranes near tigh...Continue Reading

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Citations

Nov 28, 2012·The Journal of Membrane Biology·Miwako Matsuki-FukushimaHiroshi Sugiya
Jan 19, 2016·Journal of Molecular Histology·Jiong MaXuejun Chen
Dec 28, 2010·Neuroscience Letters·Ianors IandievLeon Kohen
Jan 16, 2008·Journal of Cellular and Molecular Medicine·H SugiyaS Hashimoto
Jan 1, 2009·The Journal of Medical Investigation : JMI·Hideki IchikawaHiroshi Sugiya
Apr 2, 2009·Biochemical and Biophysical Research Communications·Nicole E RabaudJennifer M Carbrey
Nov 4, 2015·Cellular and Molecular Neurobiology·Sun Young JangTae Kwann Park
Feb 3, 2018·Caries Research·Megan WeberAlexandre R Vieira
Dec 2, 2017·Clinical and Experimental Pharmacology & Physiology·Mohamad KourghiAndrea J Yool
Feb 12, 2020·Caries Research·Amela TulekAida Mulic

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