Presence and molecular characterisation of Giardia and Cryptosporidium in alpacas (Vicugna pacos) from Peru.

Veterinary Parasitology
Hipólito Gómez-CousoSusana Pedraza-Díaz

Abstract

The presence of Giardia and Cryptosporidium was investigated in 274 faecal samples of alpacas (Vicugna pacos) from 12 herds from Peru by immunofluorescence microscopy and PCR amplification and sequencing of fragments of the ssu-rRNA and β-giardin genes from Giardia spp., as well as the ssu-rRNA gene from Cryptosporidium spp. A total of 137 samples (50.0%) were positive for Giardia spp., and 12 samples (4.4%) for Cryptosporidium spp. In ten samples (3.6%), co-infection by both pathogens was found. Herd prevalence was found to be 91.7% (11/12 herds) for Giardia and 58.3% (7/12 herds) for Cryptosporidium. Regarding the age of the animals, although Giardia was detected in animals as young as 1 week, the prevalence increased with age, reaching 80% by 8 weeks. Similarly, the highest percentage of Cryptosporidium detection (20%) was also found in the 8 week-old group. By PCR, 92 of the 274 analysed samples were positive for Giardia. Sequencing of the amplicons showed the existence of Giardia duodenalis assemblage A in 67 samples; G. duodenalis assemblage E in 24 samples; and inconsistent results between the two molecular markers used in a further sample. Cryptosporidium was only detected by PCR in 3 of the 274 samples; Cryptosporidium...Continue Reading

Citations

Apr 15, 2018·Acta Parasitologica·Alireza ZahediUna M Ryan
Nov 23, 2019·Parasitology Research·Luis A Gomez-PuertaLihua Xiao
Jan 9, 2013·Memórias do Instituto Oswaldo Cruz·Salih KukUlfet Cetinkaya

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

GraphPad Instat
SeqMan
GraphPad
MEGA
BLAST
BioEdit

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