Abstract
To investigate metallothionein (MT) expression in pterygium, pinguecula and normal conjunctiva and define its possible significance in this area of the eye. In order to further elucidate the mechanism of MT expression we correlated it with lymphocyte subpopulations (T4, T8), macrophages (CD68), Langerhans' cells (S100) and the proliferation-associated indices (PCNA, Ki67). Eighty-five surgically excised pterygia, 15 pingueculae and 20 normal conjunctivae were immunohistochemically studied by the avidin-biotin (ABC) method. A monoclonal antibody (E9) against a conserved epitope of I and II isoforms of MT was used on formalin-fixed, paraffin-embedded tissues. Statistical analysis was performed using the SPSS statistical package. Epithelial MT expression was detected in all 120 cases examined and in most of them both nuclear and cytoplasmic immunoreactivity was present. Nevertheless no statistically significant difference of MT expression was found between the three types of tissue. A statistically significant positive correlation between MT expression and lymphocyte subsets, macrophages and Langerhans' cells was found in pterygium. On the contrary, we did not find any statistical correlation in pinguecula and normal conjunctiva. ...Continue Reading
References
Jan 1, 1991·Histochemistry·H NishimuraC Tohyama
Jun 1, 1990·The Journal of Pathology·C E FullerB Jasani
Jan 1, 1989·Eye·J C Hill, R Maske
Feb 15, 1985·American Journal of Ophthalmology·A S Solomon
Apr 15, 1972·Documenta Ophthalmologica. Advances in Ophthalmology·C O Peckar
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
Aug 15, 1984·American Journal of Ophthalmology·O D PinkertonL A Shigemura
May 1, 1984·The British Journal of Ophthalmology·D J Moran, F C Hollows
Jan 1, 1994·Documenta Ophthalmologica. Advances in Ophthalmology·H R Taylor
Jan 1, 1994·Virchows Archiv : an International Journal of Pathology·D OfnerK W Schmid
Feb 1, 1995·Human Pathology·A G Douglas-JonesB Jasani
May 1, 1994·Cornea·L S Kwok, M T Coroneo
Sep 1, 1993·Histopathology·B ZelgerK W Schmid
Jan 1, 1993·Virchows Archiv. A, Pathological Anatomy and Histopathology·M FresnoM Nadji
Aug 1, 1993·Cancer·L J EttingerG D Hammond
Jan 1, 1993·Virchows Archiv. A, Pathological Anatomy and Histopathology·K W SchmidD Ofner
Jan 1, 1996·The Journal of Pathology·A AnsteyB Jasani
Nov 1, 1995·Pathology, Research and Practice·S Sunardhi-WidyaputraB Van Damme
Mar 1, 1997·American Journal of Ophthalmology·D T TanD R Smith
May 29, 1998·The Journal of Pathology·B JasaniG T Williams
Sep 17, 1999·Ophthalmic Epidemiology·S M Saw, D Tan
Oct 8, 1999·American Journal of Ophthalmology·T J Threlfall, D R English
Feb 3, 2000·The British Journal of Ophthalmology·D T TanD R Smith
Citations
Jan 1, 2004·Cornea·Pornpilas SakoonwatanyooDuncan R Smith
Feb 12, 2008·The Ocular Surface·Jeanie ChuiMinas T Coroneo
Nov 5, 2011·Clinical & Experimental Ophthalmology·Dongmei CuiKaili Wu