Presence and release of the chromogranin B-derived secretolytin-like peptide KR-11 from the porcine spleen

Regulatory Peptides
Jan DepreitereEtienne J Nouwen

Abstract

Chromogranin B (CgB) is a major matrix protein in secretory granules/large dense-cored vesicles and a precursor for smaller peptides. In an earlier study, we have identified a secretolytin-like peptide (KR-11, pCgB(637-647)) from porcine chromaffin granules. Further evidence is presented here to show the processing of chromogranin B to this peptide during axonal transport in the splenic nerve and its release in the spleen upon various conditions of stimulation. Immunohistochemical staining showed that in the porcine spleen chromogranin B and NPY completely colocalize in nerve fibres and varicosities in blood vessel walls and trabeculae, and along the loose network of smooth muscle cells in the red pulp, as identified by their alpha-smooth muscle cell actin content. No antibacterial activity was found for the porcine secretolytin-like peptide, KR-11. The change of one amino acid residue (Thr-->Asn) in the porcine homologous fragment of secretolytin appears to be responsible for its loss of antibacterial activity.

References

Aug 1, 1992·Neuroscience·H Winkler, R Fischer-Colbrie
Jun 1, 1992·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·P G HendrixM E De Broe
Jan 1, 1991·Trends in Biochemical Sciences·W B HuttnerP Rosa
Jan 1, 1990·Annals of the New York Academy of Sciences·D L BellingerD L Felten
Mar 26, 1990·FEBS Letters·T M PohlU Rüther
Jul 1, 1989·The Journal of Cell Biology·P RosaW B Huttner
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J P SimonD Aunis
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·S Natori, W B Huttner
Dec 1, 1996·The European Journal of Neuroscience·S KroesenH Winkler
Jul 3, 1999·Neuroscience·J MarksteinerH Maier
Mar 10, 2000·Regulatory Peptides·E KarlssonS Sandler

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