Presence by radioimmunoassay of a calcitonin-like substance in porcine pituitary glands

Endocrinology
B D Catherwood, L J Deftos

Abstract

We studied acidic acetone extracts of whole porcine pituitary glands for the presence of immunoreactive calcitonin (CT) using a porcine CT (pCT) RIA which did not react with other known pituitary hormones. Four preparations of porcine pituitary extract contained immunoreactive CT. Three of these displayed inhibition of binding parallel to that of authentic pCT in the pCT RIA and contained a single peak of immunoreactivity similar to pCT when studied by two different gel filtration chromatography systems. One preparation of porcine pituitary extract showed nonparallelism in RIA dose-dilution experiments and multiple immunoreactive species both similar to and larger than pCT on gel filtration in 6 M guanidine HCl. The effect of the reduction of disulfide bonds, followed by carboxymethylation of sulfhydryl groups, on immunoreactivity and apparent molecular size was similar for the CT-like substance in porcine pituitary extract and for authentic pCT. Preliminary immunohistological studies showed cytoplasmic staining in cells of the porcine adenohypophysis. These results demonstrate that the porcine pituitary gland contains a substance which has some of the immunochemical and biochemical properties of thyroidal pCT.

Citations

Jun 1, 1981·Brain Research Bulletin·J J FlynnC W Cooper
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J A FischerJ B Martin
Mar 29, 2001·Thyroid : Official Journal of the American Thyroid Association·J R HahmJ H Chung
Aug 14, 1987·Biochemical and Biophysical Research Communications·L J Deftos
Mar 1, 1982·European Journal of Cancer & Clinical Oncology·S W LambertsT J Visser
Aug 1, 1980·The American Journal of Anatomy·W B Watkins, R Y Moore
Oct 1, 1985·Journal of Endocrinological Investigation·S BalabanovaD Leupold

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