Presence, isolation and characterization of yolk DNA from chicken eggs

Science in China. Series C, Life Sciences
S LijunC Chuchu

Abstract

Yolk DNA was detected with immunocytochemistry in fertilized chicken eggs. Yolk spheres were purifield with Ficoll-400 density gradient centrifugation, followed by extraction of DNA therein. Yolk DNA is sensitive to various restriction endonucleases. but shows no obvious bands, demonstrating its sequence heterogeneity. There is only a little, if any, methylated CpG in yolk DNA. judgmg from MspI and HpII digestion. Southern blot indicated that the sequence of yolk DNA accounts for a small portion of that of genomic DNA. Its uniqueness suggests that yolk DNA may play a crucial role in early development of chickens.

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Citations

Feb 24, 2016·Scientific Reports·Vincent J MunsterRichard A Bowen

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