Presence, localization, and origin of clusterin in normal human spermatozoa.

Journal of Assisted Reproduction and Genetics
Zhijian HanWei Zhang

Abstract

Clusterin in mammalian semen is a secretory form of clusterin (sCLU) with the heterodimeric structure. It is secreted by the epididymis and seminal vesicle. It is generally agreed that clusterin mainly exists on the surface of abnormal spermatozoa and is implicated in decreased sperm motility, sperm aggregation and infertility. However, few studies observe clusterin in normal spermatozoa, which is presumed to be a novel form. Up to now, the systematical information about the presence, localization, origin and function of clusterin in normal human spermatozoa has yet not been established. The aim of our current study is to systematically research clusterin in normal human spermatozoa. We detected the presence of clusterin via western blot, explored the localization of clusterin using immunofluorescence, and investigated the origin and distribution of clusterin in human testis by western blot and immunohistochemistry. We found native clusterin in the inner plasma membrane of normal human spermatozoa. It was derived from the testis and showed similar molecular weight and heterodimeric structure compared with sCLU in semen and on the surface of abnormal spermatozoa. Clusterin in normal spermatozoa should be self-synthesized during ...Continue Reading

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Citations

Jul 31, 2013·Medical Hypotheses·Min TangChangjun Yin
Jun 18, 2014·Fertility and Sterility·Susanna McReynoldsMandy G Katz-Jaffe
Oct 30, 2015·Asian Journal of Andrology·Zhihong CuiAshok Agarwal
May 8, 2019·Reproductive Biology and Endocrinology : RB&E·Yuping GuoEnzhong Li
Mar 30, 2020·Molecular Reproduction and Development·Ewa Janiszewska, Ewa Maria Kratz
Aug 28, 2020·International Journal of Molecular Sciences·Chiara LuongoFrancisco Alberto García-Vázquez
Jun 1, 2021·Reproduction in Domestic Animals = Zuchthygiene·Cailian WangXia Lang

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