PMID: 8944638Nov 1, 1996Paper

Presence of a metabotropic and an ionotropic purinergic receptor on rat submandibular ductal cells

The American Journal of Physiology
H AmsallemJ P Dehaye

Abstract

ATP (1 microM-1 mM) increased the intracellular Ca2+ concentration ([Ca2+]i) in rat submandibular ductal cells. The dose-response curve was biphasic with a first plateau approximately 10 microM and a second increase at concentrations higher than 100 microM. This second increase was abolished in the absence of extracellular calcium or in the presence of Coomassie blue and could be mimicked by benzoyl-ATP. The activation of this response increased the uptake of extracellular manganese and the release of kallikrein, a ductal cell marker. The response at low concentrations of ATP was mimicked by ADP, 2-methylthioadenosine 5'-triphosphate (2-MeS-ATP; which was the most potent agonist), adenosine 5'-O-(2-thiodiphosphate), and UTP. Removal of extracellular calcium did not abolish this response, but ADP or 2-MeS-ATP had no effect after a preincubation with thapsigargin. A preincubation with 2-MeS-ATP desensitized the receptor involved in the response to both ADP and UTP. Similarly, a pretreatment with UTP prevented a further response to ADP. ADP increased the intracellular concentration of inositol 1,4,5-trisphosphate but did not affect the secretion of kallikrein. In conclusion, rat submandibular ductal cells possess two types of puri...Continue Reading

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Citations

Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J T TurnerB R Talamo
Jan 31, 1998·The Journal of Biological Chemistry·M G LeeS Muallem
Mar 30, 2010·Acta Physiologica·G BurnstockA Verkhratsky
Feb 3, 1999·Annals of the New York Academy of Sciences·E KabréJ P Dehaye
Jan 18, 2006·Journal of Lipid Research·Mikel Garcia-MarcosStéphanie Pochet
Jun 5, 2013·Médecine sciences : M/S·Stéphanie PochetJean-Paul Dehaye
Apr 6, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·D M ZhaoH Nakamura
Feb 22, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·I S Ambudkar
Apr 17, 2002·Neuropharmacology·S AmadioC Volonté
Jul 23, 2003·Cellular Signalling·Stéphanie PochetJean-Paul Dehaye
Jul 4, 2006·Cellular Signalling·Mikel Garcia-MarcosJean-Paul Dehaye

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