Presence of a mobilizable intracellular pool of hepatocyte growth factor in human polymorphonuclear neutrophils.

Blood
Alain GrenierMonique Dehoux

Abstract

Hepatocyte growth factor (HGF), a heparin-binding factor, is synthesized as a single-chain inactive precursor (pro-HGF), which is converted by proteolysis to an active heterodimer (mature HGF). HGF has pleiotropic activities and has been implicated in the regulation of mitogenesis, motogenesis, and morphogenesis of epithelial and endothelial cells. As polymorphonuclear neutrophils (PMNs) secrete numerous cytokines involved in the modulation of local inflammation, we investigated their ability to produce HGF. We found that HGF was stored in secretory vesicles and in gelatinase/specific granules. This intracellular stock was rapidly mobilized by degranulation when neutrophils were stimulated with phorbol myristate acetate or N-formylmethionyl-leucyl-phenylalanine. Cycloheximide did not affect the release of HGF. Moreover, HGF messenger RNA and protein expression was found in bone marrow myeloid cells, suggesting that HGF synthesis likely occurs during PMN maturation. In mature circulating PMNs, intracellular HGF was in the pro-HGF form, whereas the HGF secreted by degranulation was the mature form. Furthermore, PMNs pretreated with diisopropyl fluorophosphate only released the pro-HGF form, suggesting that PMN-derived serine prot...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·G DavidH Van den Berghe
Dec 30, 1992·Biochemical and Biophysical Research Communications·K MizunoT Nakamura
Sep 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H K WolfG K Michalopoulos
Mar 8, 1991·Cell·E Ruoslahti, Y Yamaguchi
Sep 29, 1989·Biochemical and Biophysical Research Communications·R ZarnegarG Michalopoulos
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T NakamuraA Ichihara
Jul 14, 1995·The Journal of Biological Chemistry·T F ZioncheckR J Stack
Jun 1, 1995·The Journal of Cell Biology·R Zarnegar, G K Michalopoulos
Jan 1, 1995·Immunology Today·M A Cassatella
Apr 1, 1994·Archives of Biochemistry and Biophysics·S K ChintalaC A McDevitt
May 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H SakaguchiK Kobayashi
Jan 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J Taipale, J Keski-Oja
Jan 15, 1998·Gastroenterology·D SchuppanE O Riecken
Oct 8, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L TrusolinoP M Comoglio
Feb 4, 1999·Nature Medicine·T UekiJ Fujimoto
Jan 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·F GalimiP M Comoglio

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Citations

Feb 5, 2003·The Journal of Experimental Medicine·Patrizia ScapiniMarco A Cassatella
Dec 22, 2009·Stem Cells and Development·Ali JaliliAnna Janowska-Wieczorek
Apr 27, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhijie LiC Wayne Smith
Feb 16, 2006·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Kevin ConwayWen G Jiang
Aug 30, 2003·American Journal of Respiratory and Critical Care Medicine·Sylvain Marchand-AdamBruno Crestani
Apr 19, 2006·American Journal of Respiratory Cell and Molecular Biology·Marcel BonayBruno Crestani
Oct 12, 2005·BMC Pulmonary Medicine·Marcel BonayBruno Crestani
Aug 2, 2012·Nutrients·Michael L McCaskillTodd A Wyatt
Jul 18, 2012·Proceedings of the American Thoracic Society·Bruno CrestaniMonique Dehoux
Apr 5, 2013·Seminars in Immunopathology·Sébastien JaillonAlberto Mantovani
Apr 25, 2013·Seminars in Pediatric Surgery·Kathryn J RowlandBrad W Warner
Jan 29, 2016·Journal of Immunology Research·Eileen Uribe-Querol, Carlos Rosales
Sep 25, 2004·Muscle & Nerve·Ryuichi Tatsumi, Ronald E Allen
Apr 16, 2003·European Journal of Haematology·Liang-Tsai HsiaoPo-Min Chen
Jun 23, 2009·Journal of Cellular and Molecular Medicine·Domenico Ribatti, Enrico Crivellato
Nov 1, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Kevin ConwayWen G Jiang
Dec 4, 2009·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Shoichi NishiseSumio Kawata
Jul 21, 2010·Critical Care Clinics·Jessica A Dominguez, Craig M Coopersmith
Nov 15, 2013·Journal of Gastroenterology and Hepatology·Renan Aguilar-ValenzuelaJiaren Sun
Dec 21, 2004·Critical Reviews in Oncology/hematology·Wen G JiangToshikazu Nakamura
Oct 31, 2006·The American Journal of Pathology·Zhijie LiC Wayne Smith
Feb 16, 2013·Seminars in Cancer Biology·Cristina TecchioMarco A Cassatella
Dec 6, 2014·Autoimmunity Reviews·Nicolas MolnarfiPatrice H Lalive
Aug 26, 2016·Immunological Reviews·Louise W TreffersHanke L Matlung

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