PMID: 11341962May 9, 2001Paper

Presence of a phospholipase D (PLD) distinct from PLD1 or PLD2 in human neutrophils: immunobiochemical characterization and initial purification

Biochimica Et Biophysica Acta
J M HornJulian Gomez-Cambronero

Abstract

Utilizing the transphosphatidylation reaction catalyzed by phospholipase D (PLD) in the presence of a primary alcohol and the short-chain phospholipid PC8, we have characterized the enzyme from human neutrophils. A pH optimum of 7.8-8.0 was determined. PIP(2), EDTA/EGTA, and ATP were found to enhance basal PLD activity in vitro. Inhibitory elements were: oleate, Triton X-100, n-octyl-beta-glucopyranoside, divalent cations, GTPgammaS and H(2)O(2). The apparent K(m) for the butanol substrate was 0.1 mM and the V(max) was 6.0 nmol mg(-1) h(-1). Immunochemical analysis by anti-pan PLD antibodies revealed a neutrophil PLD of approximately 90 kDa and other bands recognized minimally by anti-PLD1 or anti-PLD2 antibodies. The 90-kDa protein is tyrosine-phosphorylated upon cell stimulation with GM-CSF and formyl-Met-Leu-Phe. Protein partial purification using column liquid chromatography was performed after cell subfractionation. Based on the enzyme's regulatory and inhibitory factors, and its molecular weight, these data indicate an enzyme isoform that might be different from the mammalian PLD1/2 forms described earlier. The present results lay the foundation for further purification of this granulocyte PLD isoform.

References

Jan 1, 1991·Methods in Enzymology·M Kobayashi, J N Kanfer
Dec 22, 1989·European Journal of Biochemistry·J BalsindeF Mollinedo
Sep 14, 1995·Biochimica Et Biophysica Acta·A M Vinggaard, H S Hansen
Jun 23, 1995·The Journal of Biological Chemistry·H A BrownP C Sternweis
Jan 28, 1994·Science·S CockcroftJ J Hsuan
Oct 1, 1995·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·J Gomez-Cambronero
May 1, 1996·Trends in Pharmacological Sciences·A J MorrisM A Frohman
Apr 1, 1996·Current Opinion in Cell Biology·S SpiegelR Kolesnick
Apr 25, 1997·The Journal of Biological Chemistry·T Kodaki, S Yamashita
Jun 20, 1997·The Journal of Biological Chemistry·J H Exton
Aug 15, 1997·The Journal of Biological Chemistry·J MarcilS Bourgoin
Nov 5, 1997·Analytical Biochemistry·A J MorrisJ Engebrecht
Apr 18, 1998·The Journal of Biological Chemistry·D S MinJ H Exton
Aug 28, 1998·Cellular Signalling·J Gomez-Cambronero, P Keire
Jul 30, 1999·Biochimica Et Biophysica Acta·K E MeierK M Ella
Jul 30, 1999·Biochimica Et Biophysica Acta·L W DanielS Ghosh
Aug 4, 1999·Molecular and Cellular Endocrinology·A M StrandH S Hansen
Jun 30, 2000·Biochemical and Biophysical Research Communications·B AndrewsJ Gomez-Cambronero
Jul 14, 2000·Journal of Bacteriology·S KumariA J Wolfe
Jun 7, 2002·The Journal of Biological Chemistry·Kakuturu V N RaoKalyanasundaram Ramaswamy

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Citations

Jan 13, 2006·Oncogene·M Di FulvioJ Gomez-Cambronero
Nov 19, 2011·The Journal of Biological Chemistry·Mauricio Di FulvioJulian Gomez-Cambronero
Jun 23, 2004·European Journal of Biochemistry·Karen A CadwalladerEdwin R Chilvers
Jul 29, 2006·Blood·Nicholas LehmanJulian Gomez-Cambronero
Mar 17, 2009·Biochimica Et Biophysica Acta·Johanna Mansfeld, Renate Ulbrich-Hofmann
Feb 6, 2007·Journal of Molecular Biology·Mauricio Di FulvioJulian Gomez-Cambronero
Dec 24, 2014·Biochimica Et Biophysica Acta·Ramya GanesanJulian Gomez-Cambronero
Jan 18, 2003·Journal of Cellular Biochemistry·Alberto M MartelliL M Neri
Mar 19, 2005·Journal of Leukocyte Biology·Mauricio Di Fulvio, Julian Gomez-Cambronero
Jan 24, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nicholas LehmanJulian Gomez-Cambronero
Apr 16, 2002·The Journal of Biological Chemistry·William E HughesPeter J Parker
Apr 12, 2002·Biochemical and Biophysical Research Communications·Jen Sie Tou
Jan 18, 2003·Biochimica Et Biophysica Acta·Yoshinori Nozawa

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