PMID: 7542977Apr 1, 1995Paper

Presence of alternatively spliced-estrogen receptor mRNA variants in normal human uterine endometrium and endometrial cancer

Endocrine Journal
S HirataJ Kato

Abstract

Presence of alternatively spliced-estrogen receptor (ER) mRNA variants has been revealed in the breast cancer tissues. The ER variants transcribed from these mRNA variants were supposed to cause changes in the estrogen responsiveness of breast cancer. Although uterine endometrial cancer also has an estrogen-dependent profile, these ER mRNA variants have not yet been reported in the tumor. In the present study, we attempted to detect the exon 7 deletion- (del.7-) and exon 5 deletion (del.5) ER mRNA variants in normal human uterine endometrium (hEM) and uterine endometrial cancer tissue (hEC) by the use of reverse transcription-polymerase chain reaction-Southern blotting (RT-PCR-SB) with the PCR primers: hE4 (forward), hE6 (reverse), and hE8 (reverse), which were located in exons 4, 6, and 8, respectively. Two major products were generated from RNAs of both hEM and hEC with primers hE4 and hE8. The nucleotide sequence of the longer product was identical to exon 4-8 of human ER cDNA, whereas that of the shorter one completely deleted exon 7. Moreover, when the RT-PCR was done with the primers hE4 and hE6, the shorter product lacking exon 5 was detected with the longer one having the same sequence as exon 4-6 of human ER cDNA. Sinc...Continue Reading

Citations

Jul 31, 1997·Breast Cancer : the Journal of the Japanese Breast Cancer Society·H Iwase, S Kobayashi
Jun 1, 2002·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Luigi CobellisFelice Petraglia
Apr 9, 1999·Molecular and Cellular Endocrinology·P Monje, R Boland
Apr 12, 2002·The Journal of Steroid Biochemistry and Molecular Biology·Shuji HirataKazuhiko Hoshi
Oct 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·E C JacobsA T Campagnoni
Oct 21, 1998·The Journal of Steroid Biochemistry and Molecular Biology·K MitoH Honjo
Jul 18, 2009·Cancer Letters·Siân E TaylorFrancis L Martin

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