Presence of an additional PstI fragment in intron 1 of the chicken growth hormone-encoding gene

Gene
L MouU Kuhnlein

Abstract

A previously unreported 196-bp PstI fragment was found in intron 1 of the gene encoding chicken growth hormone (cGH) when a PCR assay for an MspI restriction fragment length polymorphism was established. A pair of PCR primers was designed according to the published cGH sequence and used to amplify a fragment which contained two MspI sites, one polymorphic and another non-polymorphic. However, amplification of genomic DNA from two strains of meat-type chickens and three strains of White Leghorn chickens yielded a PCR product which was about 200 bp larger than expected. The fragment from one of the meat-type chickens was subcloned into the vector pCR-Script SK+, and sequenced. It revealed the presence of an extra fragment of 196 bp which was flanked by the PstI sites and occurred at nt +308 of the previously reported cGH sequence.

References

Feb 1, 1993·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·N FotouhiD Zadworny
May 1, 1989·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·U KuhnleinJ S Gavora

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Citations

Jan 10, 2014·International Journal of Molecular Sciences·Yang ZhangGuohong Chen
Jun 8, 2001·Experimental Biology and Medicine·S C IpF C Leung

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