Presence of Ca(2+)-sensitive and -insensitive SM22 alpha isoproteins in bovine aorta

Biochemical and Biophysical Research Communications
T ShishiboriR Kobayashi

Abstract

Two proteins (25- and 22-kDa) that cross-react with anti-gizzard SM22 alpha antibody were isolated from bovine aorta. The former, identical to a SM22 alpha homolog reported previously (Kobayashi, R., Kubota, T., and Hidaka, H. (1994) Biochem. Biophys. Res. Commun. 198, 1275-1280), associates with the membrane fraction in the presence of Ca2+ and dissociates in the presence of EGTA, while the latter is insensitive to Ca2+. Peptide mapping and partial sequence analysis revealed that the 22-kDa protein is an isoform of the 25-kDa protein, without 22 amino acid residues from the C-terminus. Immunological analysis of the tissue distribution of these proteins showed that the 25-kDa protein exists in lung and spleen besides aorta, while the 22-kDa protein occurs specifically in the aorta and spleen.

Citations

Oct 29, 2000·Journal of Applied Physiology·Y FuJ Solway
Jan 27, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Satish Rattan, Mehboob Ali
Jul 18, 2001·Journal of Applied Physiology·K G Morgan, S S Gangopadhyay
Jun 6, 1998·Genomics·B Camoretti-MercadoJ Solway
Nov 4, 2006·Journal of Proteome Research·R Hussain ButtJens R Coorssen

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