Presence of Cryptosporidium parvum and Giardia lamblia in water samples from Southeast Asia: towards an integrated water detection system

Infectious Diseases of Poverty
Thulasi KumarVeeranoot Nissapatorn

Abstract

Access to clean and safe drinking water that is free from pathogenic protozoan parasites, especially Cryptosporidium parvum and Giardia lamblia that cause gastrointestinal illness in humans, is still an issue in Southeast Asia (SEA). This study is the first attempt to detect the aforementioned protozoan parasites in water samples from countries in SEA, using real-time polymerase chain reaction (qPCR) assays. A total of 221 water samples of 10 l each were collected between April and October 2013 from Malaysia (53), Thailand (120), the Philippines (33), and Vietnam (15). A physicochemical analysis was conducted. The water samples were processed in accordance with the US Environmental Protection Agency's methods 1622/1623.1, microscopically observed and subsequently screened using qPCR assays. Cryptosporidium oocysts were detected in treated water samples from the Philippines (1/10), with a concentration of 0.06 ± 0.19 oocyst/L, and untreated water samples from Thailand (25/93), Malaysia (17/44), and the Philippines (11/23), with concentrations ranging from 0.13 ± 0.18 to 0.57 ± 1.41 oocyst/L. Giardia cysts were found in treated water samples from the Philippines (1/10), with a concentration of 0.02 ± 0.06 cyst/L, and in untreated...Continue Reading

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Citations

Dec 1, 2009·The Science of the Total Environment·E CumminsM Cormican
Jan 10, 2021·International Journal for Parasitology·Muofhe Grace MphephuMaggy Ndombo Benteke Momba
Apr 17, 2020·International Journal of Hygiene and Environmental Health·Oluwadara Oluwaseun Alegbeleye, Anderson S Sant'Ana
Jun 22, 2021·Journal of Water and Health·Natália de Melo Nasser FavaLyda Patricia Sabogal-Paz
Jul 30, 2021·Journal of Hazardous Materials·George LukaMina Hoorfar
Jul 6, 2021·Environmental Monitoring and Assessment·Mark Raymond A VejanoWindell L Rivera

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Methods Mentioned

BETA
PCR
membrane filtration

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