Presence of duplicate genes encoding a phylogenetically new subgroup of form I ribulose 1,5-bisphosphate carboxylase/oxygenase in Mycobacterium sp. strain JC1 DSM 3803

Research in Microbiology
Sae Woong ParkYoung-Min Kim

Abstract

Ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) is the key enzyme of the Calvin reductive pentose phosphate cycle. Two sets of the structural genes for form I RubisCO were identified in Mycobacterium sp. strain JC1. The genes were clustered on the chromosome in the transcriptional order of cbbL-cbbS. Cloned cbbL-1 and cbbS-1 and cbbL-2 and cbbS-2 have open reading frames of 1431, 426, 1428, and 426 nucleotides, respectively. Primer extension analysis revealed that transcriptional start sites of cbbLS-1 and -2 genes were the nucleotides T and G located 99 and 41bp upstream of the cbbL start codons, respectively. CbbLS-1 and CbbLS-2 that were expressed in Escherichia coli exhibited RubisCO activity. A phylogeny of CbbL amino acid sequences revealed that the two enzymes in Mycobacterium sp. strain JC1 may form a new phylogenetic subgroup, type IE, in the 'red-like' group of the form I RubisCO family.

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Citations

Feb 11, 2011·Extremophiles : Life Under Extreme Conditions·Paul R NorrisLeonides A Calvo-Bado
May 15, 2013·Environmental Microbiology·Ariel Grostern, Lisa Alvarez-Cohen
Sep 2, 2015·Journal of Bacteriology·Andrew W Dangel, F Robert Tabita
Jan 27, 2012·Antonie van Leeuwenhoek·Young Min Kim, Sae Woong Park
Jul 22, 2021·Proceedings of the National Academy of Sciences of the United States of America·Eleonora ChiriChris Greening
Nov 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·Maximiliano OrtizChris Greening

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