PMID: 7540051Apr 1, 1995Paper

Presence of endogenous nicks in DNA of ejaculated human spermatozoa and its relationship to chromomycin A3 accessibility

Biology of Reproduction
G C ManicardiD Sakkas

Abstract

During spermiogenesis, mammalian chromatin undergoes replacement of nuclear histones by protamines, resulting in a DNA that is highly condensed in the mature sperm. We have previously demonstrated that a percentage of human spermatozoa exhibit 1) positivity to the guanine-cylosine-specific chromomycin A3 (CMA3) fluorochrome and 2) the presence of endogenous nicks in their DNA. In situ protamination of mature human sperm limits the percentage of sperm positive to CMA3 and exhibiting endogenous nicks. In this study, we report further investigations that aim to clarify the relationship existing between levels of CMA3 stainability and the presence of endogenous nicks in the DNA of mature human spermatozoa. Human spermatozoa from 25 different samples showed values of sensitivity to the CMA3 fluorochrome ranging from 13% to 75%. The same samples showed a percentage of sensitivity to endogenous nick translation ranging from 1% to 38%. A strong correlation (r = 0.86) was evident between these two parameters. Prior staining of sperm with the CMA3 fluorochrome drastically reduced sensitivity to nick translation. In contrast, previously nick-translated sperm stained with CMA3 showed very little difference from samples that had not been pr...Continue Reading

Citations

Mar 10, 2001·Molecular Reproduction and Development·N CaronG Boissonneault
Mar 1, 1996·Journal of Assisted Reproduction and Genetics·P G BianchiD Sakkas
May 25, 2005·Cell and Tissue Research·S E M Lewis, R J Aitken
Nov 1, 2007·Cell Biology and Toxicology·A BaumgartnerD Anderson
Jan 28, 2005·Journal of Assisted Reproduction and Genetics·H Mozdarani, A Mohseni Meybodi
Jan 14, 2011·Journal of Assisted Reproduction and Genetics·Emad Mustafa SiamMomen Hassan
Sep 10, 2011·Journal of Assisted Reproduction and Genetics·Monis Bilal ShamsiRima Dada
Aug 29, 2009·Reproductive Biomedicine Online·Stéphanie BellocYves Ménézo
Apr 30, 2009·Reproductive Biomedicine Online·Nicoletta TarozziAndrea Borini
Dec 6, 2003·Reproductive Biomedicine Online·Sergio Oehninger
Nov 6, 2004·Journal of Assisted Reproduction and Genetics·J M MorrellP V Holmes
Mar 18, 2005·Biology of Reproduction·Rémi-Martin Laberge, Guylain Boissonneault
Oct 1, 2010·Biology of Reproduction·Mirella L Meyer-FiccaRalph G Meyer
Apr 23, 2011·Obstetrical & Gynecological Survey·Mary Ellen PavoneRalph R Kazer
Sep 1, 2007·Reproductive Biology and Endocrinology : RB&E·Roxani AngelopoulouPavlos Msaouel
Oct 20, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Jill A JenkinsTerrence R Tiersch
Nov 26, 2013·Reproductive Biomedicine Online·C CoughlanT C Li
Dec 4, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Hiroyuki WatanabeYutaka Fukui
Dec 24, 2005·Asian Journal of Andrology·J ErenpreissA Giwercman
Dec 30, 2014·Advanced Biomedical Research·Farhad Golshan Iranpour
Jan 19, 2010·Fertility and Sterility·Denny Sakkas, Juan G Alvarez
Aug 24, 2011·Human Fertility : Journal of the British Fertility Society·Xiao-Dong ZhangGuo-Ning Huang
Jun 26, 2002·Human Fertility : Journal of the British Fertility Society·Christopher De Jonge
Feb 15, 2002·Human Fertility : Journal of the British Fertility Society·Denny SakkasPatricia Grace Bianchi
Jun 12, 2008·Systems Biology in Reproductive Medicine·Frédéric LeducGuylain Boissonneault
Aug 20, 2010·Systems Biology in Reproductive Medicine·Monica MuratoriElisabetta Baldi
Aug 26, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Christiane NardonChristian Biémont
Jul 17, 2012·Reproductive Biomedicine Online·Claudia RoessnerSonja Grunewald
Jan 25, 2011·Urology·Alessandro Natali, Paul J Turek
Oct 12, 2010·Theriogenology·J GosálvezJ F Moreno
Jul 30, 2010·Fertility and Sterility·Agustín García-PeiróJordi Benet
Jun 25, 2010·Urology·Rakesh K SharmaAshok Agarwal

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