Presence of esterase and laccase in Bacillus subtilis facilitates biodegradation and detoxification of cypermethrin

Scientific Reports
Saurabh GangolaParul Chaudhary

Abstract

Ubiquitous presence of cypermethrin as a contaminant in surface stream and soil necessitates to develop potential bioremediation methods to degrade and eliminate this pollutant from the environment. A cypermethrin utilizing bacterial strain (MIC, 450  ppm) was isolated from the soil of pesticide contaminated agriculture field and characterized by using polyphasic approach. On molecular basis bacterial isolate showed 98% homology with Bacillus subtilis strain 1D. Under optimized growth conditions, bacteria showed 95% degradation of cypermethrin after 15 days and the end products of cypermethrin biodegradation under aerobic conditions were cyclododecylamine, phenol, 3-(2,2-dichloroethenyl 2,2-dimethyl cyclopropane carboxylate,1-decanol,chloroacetic acid, acetic acid, cyclopentan palmitoleic acid, and decanoic acid. Amplification of esterase (700 bp) and laccase (1200 bp) genes was confirmed by PCR which showed a possible role of these enzymes in biodegradation of cypermethrin. In the presence of cypermethrin Km value(s) of both the enzymes was low than the control. A nobel cypermethrin degradation pathway followed by B. subtilis was proposed on the basis of characterization of biodegraded products of cypermethrin using GC-MS. Cyp...Continue Reading

References

Sep 1, 1988·Journal of Bacteriology·M A Mellano, D A Cooksey
Jan 1, 1996·Methods in Molecular Biology·M Bazzicalupo, R Fani
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Oct 22, 2008·Journal of Biotechnology·Luciana PereiraLígia O Martins
Oct 24, 2008·Phytopathology·Adam Driks
Nov 6, 2012·Insect Biochemistry and Molecular Biology·Theresia Estomih NkyaJean-Philippe David
Jun 29, 2013·Journal of Agricultural and Food Chemistry·Varsha P SalunkheSanjay D Sawant
Apr 1, 2014·Environmental Science & Technology·D Xanat Flores-CervantesHans-Peter E Kohler
Nov 16, 2014·Applied Microbiology and Biotechnology·Ying XiaoGuohua Zhong
Mar 19, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Shanivarsanthe Leelesh RamyaAbraham Verghese
Jan 9, 2017·Trends in Biotechnology·Ming ChenGuangming Zeng

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Citations

Dec 19, 2019·Scientific Reports·Mostafa Galal AboelkheirFernando Gomes de Souza
Apr 16, 2020·Frontiers in Microbiology·Wenping ZhangShaohua Chen
Aug 21, 2019·Frontiers in Microbiology·Pankaj BhattShaohua Chen
Feb 23, 2020·Current Microbiology·Alexis RodríguezMa Laura Ortiz-Hernández
Apr 1, 2020·Frontiers in Microbiology·Yanmei FengShaohua Chen
Apr 5, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Magdalena PodbielskaEwa Szpyrka
Oct 27, 2020·Frontiers in Microbiology·Alison G Blanton, Brittany F Peterson
Nov 10, 2020·Frontiers in Bioengineering and Biotechnology·Pankaj Kumar Arora
Mar 19, 2021·Critical Reviews in Biotechnology·Pankaj BhattShaohua Chen
Jul 25, 2020·Bioresource Technology·Pankaj BhattShaohua Chen
Mar 30, 2021·Environmental Science & Technology·Willian Garcia BirolliEdson Rodrigues-Filho
May 1, 2021·Journal of Environmental Management·Muhammad BilalShaohua Chen
Jun 1, 2021·Environmental Science and Pollution Research International·Charlene S AnjosAndré L M Porto

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Methods Mentioned

BETA
PCR
Assay

Software Mentioned

SPSS
Design Expert
MEGA
BLAST

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