Presence of galanin in human pancreatic nerves and inhibition of insulin secretion from isolated human islets

Cell and Tissue Research
B AhrénB E Dunning

Abstract

In several animal species, galanin occurs in pancreatic nerves and inhibits insulin secretion. However, the presence and action of galanin in the human pancreas have not been established. Therefore, we examined the presence and nature of human pancreatic galanin-like immunoreactive material (GLIR) and the effects of galanin on glucose-stimulated insulin secretion from isolated human islets. Immunofluorescent staining of human pancreas revealed GLIR in fine varicose fibers in both islets and exocrine parenchyma. Furthermore, acid extracts of pancreas (n = 3) and isolated islets (n = 3) contained 0.17 +/- 0.06 and 0.23 +/- 0.11 pmol GLIR/mg protein. Human pancreatic GLIR coeluted with synthetic porcine galanin from Sephadex G-50. Moreover, synthetic porcine galanin inhibited glucose-stimulated insulin secretion from collagenase-isolated human islets at dose rates greater than 10(-8) M. Thus, (1) human pancreas is innervated by galanin-containing nerves, (2) human pancreatic GLIR is of similar size as synthetic porcine galanin, and (3) porcine galanin inhibits glucose-stimulated insulin secretion from human islets. Therefore, galanin could be an important local regulator of insulin secretion in man.

References

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Citations

Jul 1, 1992·Diabetes/metabolism Reviews·W Creutzfeldt, M Nauck
May 1, 1991·Cell and Tissue Research·S LindskogF Sundler
Jul 1, 1993·Diabetologia·J J HolstH Kofod
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Mar 30, 2000·Journal of Anatomy·E T BaltazarJ Yamada
Mar 26, 2008·Pancreas·Mark E Brooke-SmithGino T P Saccone
Oct 1, 1991·Acta Physiologica Scandinavica·B E DunningB Ahrén
Oct 1, 1993·Acta Physiologica Scandinavica·B E Dunning, B Ahrén
Oct 27, 2015·Histochemistry and Cell Biology·P PodlaszJ Kaleczyc
Jan 27, 2005·Biochemical and Biophysical Research Communications·Nguyen Thi Thu QuynhClaes-Göran Ostenson
Sep 18, 2001·The Journal of Veterinary Medical Science·E T BaltazarJ Yamada
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