PMID: 9192202May 1, 1997Paper

Presence of methenamine/glutathione mixtures reduces the cytotoxic effect of sulphur mustard on cultured SVK-14 human keratinocytes in vitro

Human & Experimental Toxicology
C N SmithD G Upshall

Abstract

The basal epidermal keratinocytes of the skin are a main target for the vesicating agent, sulphur mustard (SM). A human keratinocyte cell line (SVK-14) has been used to model the effects of SM on the basal epidermal keratinocytes and subsequently to test the efficacy of potential prophylactic compounds in reducing the SM-induced cytotoxicity. The cultures were pretreated with mixtures of methenamine (HMT) and glutathione (GSH) for 1 h prior to exposure to 10 microM SM. The viability of the cultures was then assessed using neutral red (NR) dye uptake and crystal violet DNA staining assays at 24 h intervals post exposure. Pretreatment led to a 1.9 fold increase in culture viability (NR assay) compared to those exposed to SM only, and a 2.3 fold increase in cell number (crystal violet assay). Photomicrography showed that pretreatment preserved the morphology of the cultured cells and maintained their mitotic activity whereas those exposed to SM only show non-proliterative cultures with extensive cellular damage. The results of this study show that it is possible to protect mitotically active cultures from the effects of SM, however the measures must be in place prior to SM exposure.

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Citations

Feb 16, 2002·Biochemical and Biophysical Research Communications·Marc-André RaymondMarie-Josée Hébert
Oct 27, 2010·The Journal of Pharmacology and Experimental Therapeutics·Neera Tewari-SinghRajesh Agarwal
Aug 19, 2010·Annals of the New York Academy of Sciences·Jeffrey D LaskinDebra L Laskin
Nov 30, 2011·International Journal of Toxicology·F Alan Andersen
Mar 5, 2005·Journal of Applied Toxicology : JAT·Rachel Simpson, Christopher D Lindsay
Oct 3, 2019·Critical Reviews in Toxicology·Leila EtemadMahdi Balali-Mood
Jun 29, 2001·Journal of Applied Toxicology : JAT·K B AtkinsD B Hinshaw
Dec 29, 1998·Human & Experimental Toxicology·C D Lindsay, J L Hambrook
Dec 5, 1998·The American Journal of Physiology·B C SantosS R Gullans

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