Presence of Penaeus monodon densovirus in the ovary of chronically infected P. monodon subadults

Diseases of Aquatic Organisms
Sudini FernandoRapeepun Vanichviriyakit

Abstract

Shrimp infected with Penaeus monodon densovirus (PmoDNV) usually display no specific gross signs, but heavy infections can kill postlarvae and retard juvenile growth. In the present study, samples of hepatopancreas, feces, gonads and hemolymph were isolated from male and female P. monodon subadults chronically infected by PmoDNV. Each sample of hepatopancreas and gonad was divided into 2 parts: one for PmoDNV detection by polymerase chain reaction (PCR), and the other for routine histology and immunohistochemistry. The frequency of positive findings via PCR assays was 92% in the hepatopancreas, 57% in feces, 50% in ovary, 35% in hemolymph and 0% in the testis. Using the densitometric value (DV) of the specific band for PmoDNV relative to that of the β-actin gene as an index of the viral load in the samples, no significant differences were observed among sample types and sexes. Hematoxylin-eosin staining of infected hepatopancreas revealed typical PmoDNV inclusions in the nuclei of infected cells. The ovaries with high DV (>1) contained various types of inclusions along the row of the follicular cells or possibly in the connective tissue cells surrounding the oocytes. Using immunohistochemistry with specific probes to detect Pmo...Continue Reading

References

Jan 8, 2000·Diseases of Aquatic Organisms·W SukhumsirichartT W Flegel
May 30, 2002·Diseases of Aquatic Organisms·Subramaniyan ManivannanIddya Karunasagar
Jan 23, 2004·Diseases of Aquatic Organisms·Rudrappa K UmeshaIddya Karunasagar
Jul 27, 2005·Diseases of Aquatic Organisms·Sombat RukpratanpornPaisarn Sithigorngul
Sep 3, 2013·Indian Journal of Virology : an Official Organ of Indian Virological Society·Muhammed P SafeenaIndrani Karunasagar
Jan 16, 2016·Journal of Invertebrate Pathology·Chaweewan ChimwaiPongsopee Attasart

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Citations

Sep 26, 2020·Journal of Fish Diseases·Iyapa PalangRapeepun Vanichviriyakit

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