PMID: 9530875Apr 8, 1998Paper

Presence of polymerized and free forms of the non-toxic type 2 ribosome-inactivating protein ebulin and a structurally related new homodimeric lectin in fruits of Sambucus ebulus L

Planta
L CitoresT Girbés

Abstract

Mature leaves of dwarf elder (Sambucus ebulus L.) contain the non-toxic type 2 ribosome-inactivating protein ebulin 1 (Girbés et al., 1993b, J. Biol. Chem. 268: 18195-18199). We have now found that the green fruits of dwarf elder contain both free and polymerized forms of ebulin (ebulin f) and a new homodimeric D-galactose-binding lectin (SELfd). Polymerized material containing ebulin and lectin is composed of aggregates of variable relative molecular mass, some of them being close to 250,000. These aggregate forms are maintained in part by reducible disulphide bridges and reconstitute from reductant-free dialyzed material previously reduced with 2-mercaptoethanol. Direct incubation of free ebulin f with the free SELfd did not lead to polymerization, thus indicating that polymerization triggers some kind of substantial and perhaps catalyzed change in the structure of these proteins. Ebulin-containing polymerized material reacts with anti-ebulin f antibodies. Our results indicate that ebulin f is a fruit-form of ebulin 1. In contrast to green fruits, mature fruits lack both polymerized material and ebulin f, thus indicating some kind of reserve role for them in green fruits. Polymerization of ebulin and the dimeric lectin may re...Continue Reading

Citations

Dec 7, 2002·The International Journal of Biochemistry & Cell Biology·F Javier AriasTomás Girbés
Oct 15, 2013·Journal of Natural Products·Roshan R KulkarniSwati P Joshi
Feb 28, 2015·Toxins·Pilar JiménezTomás Girbés
May 27, 2015·Toxins·Joachim SchrotMatthias F Melzig
Nov 13, 2012·Toxicon : Official Journal of the International Society on Toxinology·Pilar JiménezTomás Girbés
Apr 12, 2017·Journal of Evidence-based Complementary & Alternative Medicine·Marzie JabbariMohammad Hashem Hashempur
Mar 15, 2014·International Journal of Food Sciences and Nutrition·Diana IvanovaYoana Kiselova-Kaneva
Oct 24, 2015·Cell Biology and Toxicology·Hamed FathiHamidreza Mohammadi

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