Presence of quorum-sensing systems associated with multidrug resistance and biofilm formation in Bacteroides fragilis

Microbial Ecology
Lilian PumbweHannah M Wexler

Abstract

Bacteroides fragilis constitutes 1-2% of the natural microbiota of the human digestive tract and is the predominant anaerobic opportunistic pathogen in gastrointestinal infections. Most bacteria use quorum sensing (QS) to monitor cell density in relation to other cells and their environment. In Gram-negative bacteria, the LuxRI system is common. The luxR gene encodes a transcriptional activator inducible by type I acyl-homoserine lactone autoinducers (e.g., N-[3-oxohexanoyl] homoserine lactone and hexanoyl homoserine lactone [C6-HSL]). This study investigated the presence of QS system(s) in B. fragilis. The genome of American-type culture collection strain no. ATCC25285 was searched for QS genes. The strain was grown to late exponential phase in the presence or absence of synthetic C6-HSL and C8-HSL or natural homoserine lactones from cell-free supernatants from spent growth cultures of other bacteria. Growth, susceptibility to antimicrobial agents, efflux pump gene (bmeB) expression, and biofilm formation were measured. Nine luxR and no luxI orthologues were found. C6-HSL and supernatants from Yersinia enterocolitica, Vibrio cholerae, and Pseudomonas aeruginosa caused a significant (1) reduction in cellular density and (2) inc...Continue Reading

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Citations

Dec 14, 2011·Applied Microbiology and Biotechnology·Huijuan XuYu Liu
Sep 27, 2012·Applied Microbiology and Biotechnology·Bo Jiang, Yu Liu
Aug 15, 2009·Drugs·Xian-Zhi Li, Hiroshi Nikaido
May 28, 2010·Biotechnology and Bioengineering·Huijuan Xu, Yu Liu
Sep 13, 2013·Journal of Basic Microbiology·Rafael José Marques PeixotoRegina Maria Cavalcanti Pilotto Domingues
Feb 12, 2009·FEMS Microbiology Reviews·Jose Luis MartinezCarolina Alvarez-Ortega
Dec 1, 2012·Acta Microbiologica Et Immunologica Hungarica·Zoltán G VargaJoseph Molnár
Jun 1, 2014·Biochemical and Biophysical Research Communications·Jingjing SunAixin Yan
Mar 27, 2012·FEMS Immunology and Medical Microbiology·Gianfranco DonelliPaola Mastrantonio
Mar 7, 2014·Acta Crystallographica. Section D, Biological Crystallography·Truc KimKyeong Kyu Kim
Nov 11, 2019·Archives of Microbiology·Hye-In Jang, Yong-Bin Eom
Mar 21, 2020·Microorganisms·Xihong ZhaoTian Ding
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Feb 20, 2020·Canadian Journal of Microbiology·Hye-In JangYong-Bin Eom
Sep 21, 2020·Microbial Pathogenesis·Mina YekaniMohammad Yousef Memar
Dec 15, 2010·Environmental Science & Technology·Huijuan Xu, Yu Liu

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