Presence of Recombinant Strain of Cucurbit Aphid Borne Yellows Virus in Iran

Iranian Journal of Biotechnology
Seyyed Hossein Vafaei, Maryam Mahmoodi

Abstract

Background:Cucurbit aphid-borne yellows virus (CABYV) is among the most important yellowing viruses on cucurbits in Iran. The presence of CABYV has been previously reported from the major cucurbit growing areas in Iran, however, there are few studies concerning the detection of the different strains of this virus in the grower's fields, and especially, there is no report of the weed host plants near crop fields. Objectives: This study was done in order to detect the new strains of the CABYV polerovirus in cucurbits and the weed plants in the Lorestan province, Iran, as an introductory investigation for initiating a program of the breeding for resistance. Material and Methods: During a survey carried out in 2013-2014 in Lorestan province; Iran, 189 cucurbit and 261 weed samples were investigated for the presence of CABYV using RT-PCR. In addition, the phylogeny and nucleotide similarities were discussed on the basis of the partial nucleotide sequence of RNA dependent RNA polymerase (RdRP) gene. Results: The RT-PCR carried out on leaf samples revealed the infection with the CABYV in 43 cucumber and 12 weed samples. RT-PCR using strain specific primers detected the presence of the both common (C) and recombinant (R) strains of CAB...Continue Reading

References

Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Apr 17, 2008·Archives of Virology·Hai-ying XiangJia-lin Yu
Nov 21, 2014·Advances in Virus Research·Hervé Lecoq, Nikolaos Katis

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Citations

Jul 3, 2020·Plants·Julia MinickaBeata Hasiów-Jaroszewska

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