Presence of retinal pericyte-reactive autoantibodies in diabetic retinopathy patients

Scientific Reports
Lingjun ZhangFeng Lin

Abstract

The loss of retinal pericytes (RPCs) is a hallmark of early stage diabetic retinopathy (DR), but the mechanism underlying RPC death is unclear. Although it was postulated in previous studies using bovine RPCs that autoantibodies against RPCs might develop and induce RPC death, it is unknown whether autoantibodies against cell-surface antigens on human RPCs exist in DR patients, whether such autoantibodies contribute to RPC damage/loss, and if they do, through which mechanism. We screened serum samples from DR patients and controls using primary human RPCs and found that that levels of IgGs reactive to RPCs were significantly higher in the DR group than the control group. Serum samples with higher RPC-reactive IgG levels induced more severe complement-mediated RPC damage than those with lower RPC-reactive IgG levels. We also assessed levels of the complement-activation products C3a, C4a and C5a in these serum samples, and found that serum levels of C3a and C5a, but not C4a, were higher in the DR group than control group. These data provide evidence the first time showing that autoantibodies against RPCs can develop in DR patients, and that these autoantibodies could contribute to pericyte damage through complement activation.

References

Dec 15, 1992·American Journal of Ophthalmology·A M Abu el AsrarA T Reder
Jan 1, 1986·Tissue & Cell·D E Sims
Oct 1, 1994·Immunological Reviews·M S Sandrin, I F McKenzie
May 1, 1994·Diabetic Medicine : a Journal of the British Diabetic Association·M S ArdawiA A Bahnassy
Jan 1, 1993·Fundamental & Clinical Pharmacology·C BaudouinP Gastaud
Nov 5, 1999·Retina·M A Attawia, R C Nayak
Feb 13, 2001·Clinica Chimica Acta; International Journal of Clinical Chemistry·Z TurkB Benko
Sep 28, 2002·Diabetes·Hans-Peter HammesUrban Deutsch
Mar 6, 2003·Vascular Pharmacology·G NicoloffP Christova
Jul 4, 1964·Lancet·E D POWELL, R A FIELD
Jan 15, 2004·Current Directions in Autoimmunity·Jane E Salmon, Guillermina Girardi
May 27, 2004·Investigative Ophthalmology & Visual Science·BingFen LiuRam H Nagaraj
Jul 3, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Antonia M JoussenAnthony P Adamis
Apr 1, 2005·Journal of the American Society of Nephrology : JASN·Patrick N Cunningham, Richard J Quigg
May 27, 2005·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·H-P Hammes
Oct 11, 2005·Neuro-oncology·Gabriele Bergers, Steven Song
Jul 25, 2006·Experimental Neurology·Henry J KaminskiFeng Lin
Aug 20, 2010·Nature Immunology·Daniel RicklinJohn D Lambris
Jun 4, 2011·Progress in Retinal and Eye Research·Johnny Tang, Timothy S Kern
Jul 13, 2012·Investigative Ophthalmology & Visual Science·Yan LiFeng Lin
Oct 31, 2012·Journal of Leukocyte Biology·Ramaprasad TalahalliRose A Gubitosi-Klug

❮ Previous
Next ❯

Citations

Sep 7, 2016·Journal of Diabetes and Its Complications·Nele WarmkeRichard M Cubbon
Jun 3, 2017·Vision Research·Heping Xu, Mei Chen
Jan 4, 2019·Critical Reviews in Clinical Laboratory Sciences·Xiu WangYan Zhang
Sep 19, 2018·EMBO Molecular Medicine·Isha Akhtar-SchäferThomas Langmann
Oct 16, 2019·Inflammopharmacology·Benjamin G SpencerJohn W Finnie
Aug 2, 2018·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yan LiFeng Lin
Nov 7, 2019·Proceedings of the National Academy of Sciences of the United States of America·Tae-Yun KangAndre Levchenko
Jan 12, 2021·Progress in Retinal and Eye Research·Warren W PanPatrice E Fort
Aug 11, 2019·Molecular Immunology·Ramzi A Ajjan, Verena Schroeder

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
flow cytometry
Cytometric

Software Mentioned

FlowJo
FCAP Array TM
JMP9

Related Concepts

Related Feeds

Alternative Complement Pathway

The Alternative Complement Pathway is part of the innate immune system, and activation generates membrane attack complexes that kill pathogenic cells. Discover the latest research on the Alternative Complement Pathway.

Antibodies: Complement Activation

The complement system can be activated by antigen-associated antibody. In the classical pathway of complement activation, C1q, C4b, and C3b are all able to bind to the Fc portion of IgG or IgM. Find the latest research on antibodies and complement activation here.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Related Papers

The British Journal of Ophthalmology
F de Oliveira
Nihon rinsho. Japanese journal of clinical medicine
Shinji FujinoIkunosuke Sakurabayashi
Nihon rinsho. Japanese journal of clinical medicine
Y Kuroda, Ikunosuke Sakurabayashi
© 2021 Meta ULC. All rights reserved