Presence of salmon gonadotropin-releasing hormone (GnRH) and compounds with GnRH-like activity in the ovary of goldfish

Endocrinology
Debananda Pati, Hamid R Habibi

Abstract

The presence of ovarian GnRH and/or compounds with GnRH-like activity was investigated in the goldfish ovary. Goldfish ovary was extracted using an acetone/HCl/ether mixture and was purified by Waters C-18 Sep-Pak columns (ovarian extract). The goldfish ovarian extract was found to 1) stimulate gonadotropin release and subunit messenger RNA production in the goldfish pituitary that was inhibited by a GnRH antagonist; 2) stimulate germinal vesicle breakdown in the prophase-I arrested follicle-enclosed goldfish oocytes in vitro, which was inhibited by a GnRH antagonist; 3) immunoreact with various GnRH antisera; and 4) bind to GnRH receptors in the goldfish pituitary and ovary as well as rat pituitary. Further purification with HPLC revealed the presence of two compounds with GnRH-like activity. One with identical chromatographic characteristics, amino acid composition, and primary structure to that of the salmon GnRH (sGnRH), and the other a novel compound with GnRH-like activity.

Citations

Feb 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·M M Di FioreA D'Aniello
May 4, 2001·Molecular Reproduction and Development·S UzbekovaJ J Lareyre
Jan 5, 2011·General and Comparative Endocrinology·Rosanna ChianeseGilda Cobellis
Nov 23, 2007·General and Comparative Endocrinology·George E BentleyKazuyoshi Tsutsui
Mar 3, 2015·General and Comparative Endocrinology·K P Joy, R Chaube
May 18, 1999·Biology of Reproduction·K R von SchalburgN M Sherwood
Aug 28, 2007·Molecular Reproduction and Development·Josephine N Nocillado, Abigail Elizur
Oct 16, 2007·Molecular Reproduction and Development·Anamika SenguptaRajagopala Sridaran
Jan 10, 2001·Canadian Journal of Physiology and Pharmacology·C V Andreu-Vieyra, H R Habibi
Jan 10, 2001·Canadian Journal of Physiology and Pharmacology·D PatiH R Habibi
Jun 12, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·S UzbekovaB Breton
Apr 10, 2012·General and Comparative Endocrinology·David A Irwin, Glen Van Der Kraak

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