Presence of Segmented Filamentous Bacteria in Human Children and Its Potential Role in the Modulation of Human Gut Immunity

Frontiers in Microbiology
Bo ChenCharlie Xiang

Abstract

Segmented filamentous bacteria (SFB) are commensal organisms that grow by anchoring a specialized holdfast structure to the intestinal walls of a variety of animals. Interaction of SFB with Peyer's patches in mice promotes the post-natal maturation of the immune system. We previously reported that the colonization of SFB in humans mainly occurs by 36 months of age, and is difficult to be detected afterward. In this study, we measured the level of SFB in intestinal fluids of human children. SFB were found via qPCR to represent a small fraction of the whole SFB-positive microbiota (105 SFB in 1011 total bacteria). Bacteria with filamentous segmented morphology were observed in intestinal fluids via fluorescent in situ hybridization, and from gut biopsies via scanning electron microscopy. SFB-specific DNA and peptide fragments were also identified via multiple displacement amplification PCR and mass spectrometry. There was an overall positive correlation between the presence of SFB and the titer of total secretory immunoglobulin A (sIgA), which is more apparent in intestinal fluids of the age group of 8-36 months. Afterward there was a decline of SFB in numbers correlated with a reduction of total sIgA. RT-qPCR analysis of the ter...Continue Reading

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Citations

Jun 5, 2019·International Journal of Molecular Sciences·Giovanni Brandi, Giorgio Frega
Jan 29, 2020·European Journal of Immunology·Gillian Fitzgibbon, Kingston H G Mills
Apr 12, 2020·Journal of Applied Microbiology·Xin MengHao Wu
Apr 16, 2019·Frontiers in Immunology·Hong-Yu ChengWen-Tao Ma
Dec 11, 2019·Nature Microbiology·Iris NkambaPamela Schnupf
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Datasets Mentioned

BETA
AP012209.1
GSE68425
SRP069299

Methods Mentioned

BETA
PCR
scanning electron microscopy
biopsies
ELISA
biopsy
chips
MDA
sedation
electrophoresis
Fluorescence

Software Mentioned

Primer3
Sequest HT
Agilent Genespring GX
BLAST
Casava
Feature Extraction
Proteome Discoverer
GeneSpring
GraphPad Prism

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