Presence of synaptonemal complex protein 1 transversal filament-like protein in human primary spermatocytes

Human Reproduction
A PousetteC Höög

Abstract

The synaptonemal complex (SC) is involved in the pairing of chromosomes during meiosis. We found that antibodies raised against a protein component (P1) of the mouse synaptonemal complex, mouse SCP1, also identified the SC in human primary spermatocytes. Biopsies from 18 men presented with infertility were evaluated by light-field microscopy and grouped into five categories: normal spermatogenesis, Sertoli cell-only syndrome, meiotic disturbances, spermiogenic (i.e. differentiation) disturbances, and other combined disturbances. In all the normal subjects the SCP1 antibody distinctly stained the synaptonemal complexes of primary spermatocytes, whereas Sertoli cells, spermatogonia or spermatids were never stained. In three of the groups, which had germ cells but showed spermatogenic disturbances, the staining was similar to that seen in normal subjects. In sharp contrast to this, in sections from men with Sertoli cell-only syndrome no specific staining was seen. This study demonstrates that a SCP1-related protein is also conserved in the synaptonemal complex in meiotic cells from man. Further studies will reveal to what extent the absence or the non-functionality of SCP1 contributes to male infertility.

Citations

Apr 28, 2000·Fertility and Sterility·S J SilberA Van Steirteghem
Jul 22, 2005·Nature Reviews. Cancer·Andrew J G SimpsonLloyd J Old
Mar 1, 2003·Applied Immunohistochemistry & Molecular Morphology : AIMM·Evgeny YakirevichMurray B Resnick
Apr 28, 2010·Expert Opinion on Biological Therapy·Morten Frier GjerstorffHenrik Jorn Ditzel
Aug 19, 2008·Fertility and Sterility·Carme NoguésCristina Templado
Feb 26, 2000·Annual Review of Genetics·D Zickler, N Kleckner
Jul 17, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·H StoopL Looijenga
Jul 15, 2015·Oncotarget·Morten F GjerstorffHenrik J Ditzel

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