Presence of thyrotropin receptor in infant adipocytes

Pediatric Research
A JansonClaude Marcus

Abstract

In healthy infants, the levels of TSH are known to peak at 50-100 times adult values during the first days of life. In studies of isolated human infant adipocytes, we have earlier shown that bovine TSH (bTSH) has a strong lipolytic effect, accompanied by a blunted response of adipocytes to catecholamines. In this study, we used human recombinant TSH (hTSH), and incubation of adipocytes with hTSH induced a lipolytic response similar to that obtained with the beta-adrenergic receptor agonist isoprenaline in adipocytes isolated from three infants. The lipolytic effect of hTSH was completely blocked by inhibitory TSH receptor (TSHR) antibodies. The TSHR mediates the effects of TSH in the thyroid, and it has been detected in some extrathyroid tissues, but not in isolated human adipocytes or childhood adipose tissue. In this study, we found TSHR RNA in infant and adult adipose tissues and isolated adipocytes with reverse transcriptase-PCR. The sequence of the amplified PCR product agreed with the published sequence. Northern blot hybridization on RNA prepared from infant adipose tissue showed a transcript of the expected size, and the expression of TSHR seemed higher in infant than in adult adipose tissue. In conclusion, this study i...Continue Reading

References

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Citations

Mar 6, 2002·Clinical and Experimental Immunology·M Ludgate, G Baker
Jan 9, 2008·The Journal of Endocrinology·Min Lu, Reigh-Yi Lin
Sep 10, 2004·The Journal of Clinical Endocrinology and Metabolism·Ashraf F KamelClaude Marcus
Sep 13, 2002·American Journal of Physiology. Cell Physiology·Andrea BellAlexander Sorisky
Jul 27, 2000·American Journal of Physiology. Cell Physiology·A BellA Sorisky
Aug 26, 2006·Nature Clinical Practice. Endocrinology & Metabolism·Andreas SchäfflerChrista Buechler
Dec 16, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Andrea BellAlexander Sorisky
Apr 16, 2010·European Journal of Endocrinology·Ferruccio SantiniAldo Pinchera
Apr 27, 2021·The Journal of Clinical Endocrinology and Metabolism·Maxime M BosDiana van Heemst
May 26, 2009·Progress in Lipid Research·Max Lafontan, Dominique Langin
Oct 23, 2009·Metabolism: Clinical and Experimental·Annemarie GagnonAlexander Sorisky

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