Preservation of caprine preantral follicle viability after cryopreservation in sucrose and ethylene glycol

Cell and Tissue Research
R R SantosR van den Hurk

Abstract

Caprine preantral follicles within ovarian fragments were cryopreserved in the absence or presence of 0.5 M sucrose with or without 1 M dimethyl sulfoxide and/or 1 M ethylene glycol (EG). After being thawed, they were washed in minimum essential medium with or without 0.3 M sucrose. Histological analysis of follicle integrity immediately after cryopreservation showed consistent beneficial effects of including sucrose in the three cryoprotectant solutions analyzed when tissue was thawed without sucrose (53.9+/-14.8-82.4+/-3.2% normal vs 27.6+/-1.6-36.6+/-6.5%, P<0.05). However, in further studies, the addition of sucrose to the thaw solutions proved detrimental or of no benefit. An analysis of the cryopreserved material with calcein-AM and ethidium homodimer (markers for living and dead cells, respectively) gave comparable results to those obtained by histology. Follicles cryopreserved in EG, EG plus sucrose, or sucrose alone were cultured in vitro for 24 h following warming. During this culture period, viability fell most rapidly in material cryopreserved in sucrose alone and was no longer correlated with either the viability or integrity estimates made immediately after warming. By contrast, the viability of follicles cryopres...Continue Reading

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Citations

Mar 4, 2011·Reproduction, Fertility, and Development·J R FigueiredoR R Santos
Feb 5, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·V von SchönfeldtB Sonntag
Oct 30, 2012·Zygote : the Biology of Gametes and Early Embryos·F Marco-JiménezJ S Vicente
Jun 13, 2008·Cell and Tissue Research·Regiane R SantosRob van den Hurk
Oct 20, 2007·Animal Reproduction Science·Juliana Jales de Hollanda CelestinoJosé Ricardo de Figueiredo
Jan 16, 2018·Reproduction, Fertility, and Development·N J DonfackA P R Rodrigues
Jun 22, 2012·Zygote : the Biology of Gametes and Early Embryos·Rafael RossettoJosé Ricardo de Figueiredo
May 26, 2018·Journal of Assisted Reproduction and Genetics·Anniek BusPeter E J Bols
Mar 11, 2016·Reproductive Medicine and Biology·Lalitha Devi, Sandeep Goel
Jul 3, 2021·International Journal of Molecular Sciences·Cesar Augusto Roque-BordaEduardo Festozo Vicente

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