Preservation of histological structure of cells in human skin presenting mummification and corification processes by Sandison's rehydrating solution

Forensic Science International : Synergy
Federica ColliniRiccardo Zoja

Abstract

To overcome the difficulties of construction and interpretation of microscopic material from corpses presenting mummification and corification processes, a variety of techniques and tricks are used: in this research the results of applying the Sandison's rehydrating solution are presented, generally used in archeological field on Egyptian mummies of different ages, in human cadaveric material in an advanced state of decomposition. Nineteen skin specimens were taken from corpses presenting corification and mummification processes, discovered in a time ranging between one and four months and exhumed after 11 years. Each biological sample was divided into two parts: one, directly fixed in buffered formalin 10%; the other, preliminarily treated with the Sandison's rehydrating solution and, therefore, post-fixed in 10% buffered formalin. All samples were then carried out the routine histological preparation, and the sections were stained by hematoxylin-eosin and by other histochemical stains. Under the microscope, the samples placed directly into formalin, showed marked structural changes of the various components, while those previously rehydrated with the Sandison's rehydrating solution allowed the clear recognition of different s...Continue Reading

References

Feb 1, 1989·Forensic Science International : Synergy·E GirelaE Villanueva
Apr 1, 1967·Medical History·A T SANDISON
Jun 1, 1968·Archives of Dermatology·L Giacometti, B Chiarelli
May 1, 1997·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·G GrupeJ Soika
Nov 1, 1955·Stain Technology·A T SANDISON
May 22, 2004·Journal of the American Academy of Dermatology·Eve Judith Lowenstein
Apr 5, 2005·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·A-M Mekota, M Vermehren
Sep 27, 2005·Forensic Science International : Synergy·D BreitmeierW J Kleemann
Apr 15, 2006·Journal of Neurology, Neurosurgery, and Psychiatry·M SpitzE R Barbosa

Citations

Sep 9, 2015·BioMed Research International·Despina MoissidouRaffaella Bianucci
Jan 26, 2020·International Journal of Legal Medicine·Guendalina GentileRiccardo Zoja
Nov 20, 2016·Journal of Forensic Sciences·Chun-Chieh ChenSheng-Meng Wang

Related Concepts

Fixatives
Formalin
Microscopy
Mummies
Livor Mortis
Skin
Rehydration Solutions
Forensic Pathology Discipline
Cadaver
Eosine Yellowish

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