PMID: 5890139Sep 10, 1965

Preservation of mammalian cells in a chemically defined medium and dimethylsulfoxide

B L Brown, S C Nagle


Three established mammalian cell lines (cat kidney, L, and HeLa cells), grown in suspension in a protein-free, chemically defined medium, were stored under liquid nitrogen in the defined medium fortified with 4 and 8 percent dimethylsulfoxide. After storage for 1 month the recovery viability was 86 percent, and cells showed normal growth upon reinoculation into the defined growth medium.


Oct 7, 1966·Annals of the New York Academy of Sciences·J C Bryant
Mar 15, 1967·Annals of the New York Academy of Sciences·E Gerhards, H Gibian
Aug 1, 1970·The Annals of Thoracic Surgery·D W JirschC M Couves
Sep 1, 1968·Die Naturwissenschaften·E Gerhards, H Gibian

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