Preservation of Microvascular Integrity in Murine Orthotopic Tracheal Allografts by Clopidogrel

Transplantation
Christian HeimStephan M Ensminger

Abstract

Survival after lung transplantation is mainly limited by the development of chronic lung allograft dysfunction (CLAD). The aim of this study was to investigate if platelet inhibition by clopidogrel has a functionally relevant influence on the microvascular integrity of orthotopic tracheal allografts as an anatomic basis for the development of CLAD. We orthotopically transplanted C57Bl/6 (H-2) tracheas into CBA.J (H-2) recipients who afterwards received clopidogrel (1 mg/kg). Morphometric analysis was performed by measuring epithelial height in proportion to thickness of the lamina propria (epithelium-lamina propria ratio). Tissue oxygenation was determined using a fluorescence quenching technique, and graft perfusion monitoring was performed by laser Doppler flowmetry and lectin-binding assay. Immunohistochemistry was used for detection of CD31 and inducible nitric oxide synthase while iron deposition was shown with Prussian blue reaction. Quantitative reverse transcription polymerase chain reaction analysis was used for gene expression analysis. Isografts maintained good oxygenation and perfusion throughout the experiment, while both were drastically reduced in allografts. Treatment with clopidogrel attenuated graft hypoxia an...Continue Reading

References

Aug 1, 1997·Chest·M A BazC A Piantadosi
Mar 19, 2002·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Marc EstenneSamuel Yousem
May 23, 2002·Transplantation·Eric M GendenLloyd Mayer
Sep 18, 2003·Nature Reviews. Cancer·Gregg L Semenza
Feb 14, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Félix G FernándezT Mohanakumar
May 12, 2004·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Heyman LuckrazJohn Wallwork
Sep 17, 2004·Transplantation·Binnaz Handan OzdemirMehmet Haberal
Mar 11, 2005·The New England Journal of Medicine·Marc S SabatineUNKNOWN CLARITY-TIMI 28 Investigators
Mar 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tomohiro MurakawaMark R Nicolls
Nov 22, 2005·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Christopher PughAndrew J Ghio
May 9, 2006·The Journal of Thoracic and Cardiovascular Surgery·Silke AbeleStephan M Ensminger
Jun 24, 2006·Platelets·P SaviJ M Herbert
Feb 28, 2007·Microsurgery·Sonja SchrepferMarc P Pelletier
May 25, 2007·Archives of Physiology and Biochemistry·Alexander V OvechkinAndrew M Roberts
Oct 12, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·C N MorrellW M Baldwin
Dec 7, 2007·The Journal of Clinical Investigation·Ashok N BabuMark R Nicolls
Jun 19, 2008·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·S M ParkerA J Fisher
Mar 27, 2010·American Journal of Respiratory and Critical Care Medicine·Gundeep S DhillonMark R Nicolls
May 29, 2010·American Journal of Respiratory and Critical Care Medicine·C Ashley Finlen CopelandScott M Palmer
Aug 13, 2011·Transplantation·Geert M VerledenBart M Vanaudenaerde
Dec 21, 2011·The Journal of Physiology·Karen Y Stokes, D Neil Granger
Aug 7, 2012·Genes & Cancer·Bryan L KrockM Celeste Simon
Sep 25, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Mohammad A KhanMark R Nicolls
Aug 21, 2013·Transplant International : Official Journal of the European Society for Organ Transplantation·Raimund H M PreidlStephan M Ensminger
Mar 29, 2014·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Christian HeimStephan M Ensminger
Apr 11, 2015·Annals of the Rheumatic Diseases·Jingang HuangJörg H W Distler
Mar 12, 2016·Current Opinion in Organ Transplantation·Mark R NicollsXinguo Jiang
Oct 25, 2016·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Roger D YusenUNKNOWN International Society for Heart and Lung Transplantation
Dec 20, 2017·The Thoracic and Cardiovascular Surgeon·Christian HeimStephan Ensminger

❮ Previous
Next ❯

Citations

Apr 3, 2020·Journal of Translational Medicine·Mohammad Afzal KhanDieter Clemens Broering
Feb 8, 2021·Interactive Cardiovascular and Thoracic Surgery·Christian HeimStephan M Ensminger

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Related Papers

Transplant International : Official Journal of the European Society for Organ Transplantation
Raimund H M PreidlStephan M Ensminger
Experimental and Molecular Pathology
Alexander S FarivarMichael S Mulligan
Proceedings of the National Academy of Sciences of the United States of America
Mohammad A KhanMark R Nicolls
© 2022 Meta ULC. All rights reserved