Preservation of mitochondrial structure and function after cardioplegic arrest in the neonate using a selective mitochondrial KATP channel opener

The Annals of Thoracic Surgery
Lixing WangChristopher A Caldarone

Abstract

Mitochondrial dysfunction may contribute to early postoperative neonatal heart dysfunction. Diazoxide, a mitochondrial-selective adenosine triphosphate-sensitive potassium-channel opener, is associated with mitochondrial preservation after cardioplegic arrest. We evaluated the mitochondrial-protective effect of diazoxide in terms of mitochondrial structure and function after neonatal cardioplegic arrest. Newborn piglets (age, approximately 14 days) underwent cardiopulmonary bypass and 60 minutes of cardioplegic arrest using cold crystalloid cardioplegic solution (CCP, n = 5) or cold crystalloid cardioplegic solution with diazoxide (CCP+D, n = 5). After 6 hours of recovery, myocardium was harvested. Control myocardium from piglets that did not undergo cardiopulmonary bypass (non-CPB, n = 5) was obtained. Cardioplegic arrest was associated with translocation of Bax to the mitochondria, which was not prevented by diazoxide. Nevertheless, by electron microscopy, CCP-associated remodeling of mitochondrial structure was subjectively diminished in CCP+D hearts. In addition, CCP-associated mitochondrial permeabilization and cytochrome c release into the cytosol were prevented with CCP+D (p < 0.05). In vitro oxygen consumption of isolat...Continue Reading

References

Aug 28, 1998·Science·D R Green, J C Reed
May 25, 1999·Journal of Molecular and Cellular Cardiology·G F Baxter, D M Yellon
Feb 15, 2001·American Journal of Physiology. Cell Physiology·P KorgeJ N Weiss
Jan 10, 2002·Developmental Cell·Luca ScorranoStanley J Korsmeyer
Jan 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Cevher OzcanAndre Terzic
Mar 13, 2002·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Hidetaka WakiyamaJames D McCully
May 9, 2002·The Annals of Thoracic Surgery·Joachim P SchmittHermann Aebert
Dec 4, 2002·Biochimica Et Biophysica Acta·Florence AppaixValdur A Saks
Jan 8, 2003·The Journal of Cell Biology·Jean-Ehrland RicciDouglas R Green
Mar 20, 2003·The Annals of Thoracic Surgery·James D McCullySidney Levitsky
Dec 23, 2003·The Journal of Thoracic and Cardiovascular Surgery·Richard A JonasJane W Newburger
Dec 25, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Andrey V KuznetsovErich Gnaiger
Feb 6, 2004·The Journal of Thoracic and Cardiovascular Surgery·Mohsen KarimiChristopher A Caldarone
Jul 10, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Anthony J RousouJames D McCully
Sep 1, 2004·The Annals of Thoracic Surgery·Christopher A CaldaroneThomas D Scholz

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Citations

Jan 9, 2008·The Journal of Thoracic and Cardiovascular Surgery·Norihiko OkaChristopher A Caldarone
Mar 11, 2008·The Journal of Thoracic and Cardiovascular Surgery·Norihiko OkaChristopher A Caldarone
Oct 1, 2008·Acta Anaesthesiologica Scandinavica·S MagnasonS Gudmundsson
Nov 12, 2014·World Journal of Gastroenterology : WJG·Mateus Antunes NogueiraMarcel Cerqueira Cesar Machado
Sep 27, 2020·The Journal of Thoracic and Cardiovascular Surgery·Alejandro Suarez-PierreJennifer S Lawton

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