Preservation of nasopharyngeal smears for fluorescent antibody detection of Bordetella pertussis.

Journal of Clinical Microbiology
P P HarrisR M McKinney

Abstract

Smears from throat and nasal washings seeded with Bordetella pertussis were either treated with aprotinin (a protease inhibitor) or fixed with 1 or 10% Formalin. These smears were stored at 23, 4, and -70 degrees C. Smears were removed and stained with a fluorescent antibody conjugate for B. pertussis at intervals during 22 days of storage. Results indicate that treatment of smears with 4 or 8 U of aprotinin per ml preserved fluorescent antibody staining qualities of B. pertussis for 22 days; fixation with either concentration of Formalin was unsatisfactory.

References

Feb 1, 1957·Pediatric Clinics of North America·H M FELTON

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Citations

Jan 1, 1990·Journal of Clinical Microbiology·S A HalperinA J Wort
Apr 1, 1989·Journal of Clinical Microbiology·S A HalperinA J Wort

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