Preservation of pancreas tissue during cold storage assessed by X-ray microanalysis

American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
Inna Kozlova, G M Roomans

Abstract

Clinical transplantation requires cold storage of tissue for several hours. We have examined the elemental content in exocrine and endocrine cells in mouse pancreas after cold storage by X-ray microanalysis, and in parallel carried out morphological studies. Tissue was stored at 4 degrees C for 4-12 h in Normal Krebs-Ringer's (high Na+/K+ ratio), Modified Krebs-Ringer's (low Na+/K+ ratio), Euro-Collins, University of Wisconsin (UW) solution, and seven modified version of UW solution. Incubation in Normal Krebs-Ringer's solution caused significantly increased Na and decreased K concentrations in contrast to incubation in other solutions. The cellular concentration of Na and Cl followed the concentration in the storage solution. Changes in the endocrine cells were similar to, but less pronounced than those in exocrine cells. Calcium was retained best in UW and some variants of UW, and least in Euro-Collins. This may indicate differences in preservation of secretory granules. Also, morphological studies showed that endocrine cells were less affected than exocrine cells. In conclusion, the only factor determining the intracellular concentration of diffusible ions after cold tissue storage is the ionic composition of the extracellul...Continue Reading

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Citations

Jan 31, 2007·Microscopy Research and Technique·Ana VeríssimoTeresa Pinheiro

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