PMID: 6159279Jan 1, 1980

Preservation of red blood cells with purines and nucleosides. I. Changes of ATP, 2,3-P2G and further parameters of metabolism in red cells stored as whole blood and as resuspension at 4 degrees C and 25 degrees C

Folia haematologica : internationales Magazin für klinische und morphologische Blutforschung
D StraussC H de Verdier

Abstract

CPD blood with addition of adenine in 0.25 mM final concentration alone or in combination with guanosine and inosine each in 0.5 mM concentration and also with a higher adenine concentration--0.75 mM--was stored 6 weeks at 4 degrees C. In a second experiment red cell concentrates were prepared from CPD blood and resuspended in the same volume of a glucose-sucrose-citrate solution with the addition of adenine or guanine and both in combination with inosine. The resuspended cells were stored 7 days at 25 degrees C. Adenine and guanosine added simultaneously were most effective on the maintenance of ATP and P2G content. When guanosine was replaced by the same amount of inosine, no effect on P2G content was found in comparison with the addition of only adenine, however, the ATP content decreased more rapidly. The reason for this difference can be due to a different affinity of the IMP/GMP: pyrophosphate phosphoribosyltransferase to their substrates as well as regulation principles in the P2G bypass. The guanosine effect on red cell preservation consists in improved viability of red cells in vivo, increased nucleotide synthesis, increased formation of 2.3-P2G, delayed disc-spher transformation of red cells and decreased hemolysis. T...Continue Reading

Related Concepts

Adenosine Triphosphate, Chromium Ammonium Salt
Blood Glucose
Blood Preservation
Diphosphoglyceric Acids
Erythrocytes
Hydrogen-Ion Concentration
Nucleosides
Purines
2,3-Diphosphoglycerate, (D)-Isomer

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