Preservation of skin free-flap using trehalose

The Journal of Surgical Research
A K KitaharaY Nishimura

Abstract

In this study, we looked for a solution able to preserve traumatically amputated tissues such as nose tip, ear, and scalp for at least 48 hr that was easy to handle and low in price. Trehalose is a nonreducing disaccharide that can protect many prokaryotes, fungi, and yeasts against adverse conditions by stabilizing cell membranes. In order to study its protective effect, 60 skin free-flaps of the ears of rabbits were preserved in Euro-Collins (EC) solution or in modified Euro-Collins solution in which 7% trehalose replaced glucose (EC + 7%T) for 24, 48, and 72 hr at 4 degrees C. After completion of preservation, these flaps were transferred to the other ear with microvascular anastomosis. After 7 days, we obtained a survival rate of 100% of flaps after preservation for 24 hr in both solutions. But, after preservation for 48 hr or longer, this decreased to 60% (6 of 10 preserved flaps survived) in EC solution and 90% (9 of 10 flaps) in EC + 7%T solution. This difference became clearer after 72 hr of preservation, when the survival rate was higher and light microscopic observation showed less damage for flaps preserved in EC + 7%T solution (20% in EC and 70% in EC + 7%T) (P < 0.05). Thus, EC + 7%T solution was superior to EC sol...Continue Reading

Citations

Feb 22, 2011·Journal of Pharmaceutical Sciences·Satoshi Ohtake, Y John Wang
Jul 11, 1998·British Journal of Plastic Surgery·A K KitaharaY Nishimura
Sep 23, 1997·Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery·C W ZhanY Nishimura
Jul 31, 1999·Transplantation·T FukuseH Wada
Jun 18, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A B RichardsM Kurimoto

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