Preservation of the metaproteome: variability of protein preservation in ancient dental calculus

Science and Technology of Archaeological Research
Meaghan MackieCamilla F Speller

Abstract

Proteomic analysis of dental calculus is emerging as a powerful tool for disease and dietary characterisation of archaeological populations. To better understand the variability in protein results from dental calculus, we analysed 21 samples from three Roman-period populations to compare: 1) the quantity of extracted protein; 2) the number of mass spectral queries; and 3) the number of peptide spectral matches and protein identifications. We found little correlation between the quantity of calculus analysed and total protein identifications, as well as no systematic trends between site location and protein preservation. We identified a wide range of individual variability, which may be associated with the mechanisms of calculus formation and/or post-depositional contamination, in addition to taphonomic factors. Our results suggest dental calculus is indeed a stable, long-term reservoir of proteins as previously reported, but further systematic studies are needed to identify mechanisms associated with protein entrapment and survival in dental calculus.

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Mar 28, 2018·Nature Ecology & Evolution·Jessica HendyMatthew J Collins
Aug 31, 2018·American Journal of Physical Anthropology·Alessandra SperdutiLuca Bondioli
Nov 22, 2018·Nature Communications·Rosa R Jersie-ChristensenJesper V Olsen
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Feb 18, 2021·Journal of Proteome Research·Karren S PalmerMichael Buckley
Aug 28, 2021·Methods : a Companion to Methods in Enzymology·Manasij Pal Chowdhury, Michael Buckley

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Datasets Mentioned

BETA
PXD001362
PXD001360

Methods Mentioned

BETA
deamidation
acetylation
protein assay

Software Mentioned

Power
Proteowizard
SPSS
PATRIC
BLASTp
Mascot

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